CREAMS/ SODIUM LAURETH SULFATE BY HEAD SPACE GAS CHROMATOGRAPHIC TECHNIQUE INTRODUCTION 1-4‚ DIOXANE 1‚4-Dioxane is a heterocyclic organic compound‚ classified as an ether. It is a colorless liquid with a faint sweet odor similar to that of diethyl ether. The compound is often called simply Dioxane because the other Dioxane isomers (1‚2- and 1‚3-) are rarely encountered. CHEMISTRY OF 1-4‚ DIOXANE
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The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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Discussion/Conclusion In this experiment‚ t-pentyl chloride was synthesis by reacting concentrated hydrochloric acid using nucleophilic substitution sn1. In the substitution reaction the Hydrogen in HCl protonated the alcohol group in t-pentyl alcohol and turned it to a good leaving group (H2O). a tertiary carbon cation was formed. a nucleophilic attach of the negative Cl- attacked the carbon cation forming t-pentyl chloride [7]. As the reaction proceeded‚ extraction and distillation techniques were used
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The purpose of this lab was to use a calorimeter to learn when energy is being released or absorbed in a chemical reaction. My lab partner and I had to determine the heat exchanged when solid magnesium hydroxide is mixed with solid citric acid‚ using a coffee cup calorimeter. Given that citric acid is soluble in water is the property that makes this reaction possible. The first law of thermodynamics and Hess’s law are also present within this experiment. Knowing the difference between endothermic
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Amino acids are important building blocks; they play an essential role in the formation of proteins and as intermediates in metabolism. Amino acids can serve as cell signaling molecules and can regulate gene expression. An optimal amount of amino acids is essential for health. There are over 500 amino acids‚ however only 20 are commonly found. Amino acids all have an amine functional group and a carboxylic acid functional group. They differentiate from each other in their side chains. Only 10 of
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UNIVERSITY OF WATERLOO Department of Chemistry Synthesis of ACETAMINOPHEN Laboratory Report 1 CHEM 123L January 19‚ 2014 Prepared for: Dr. S. Stathopulos Department of Sciences‚ Chemistry Faculty University of Waterloo Teaching Assistant: Aaron Chan Prepared by: Section 001 Vithurjan Satkunarasan 20495383 Lab Partner: Ken Xu Introduction The synthesis of Acetaminophen is based on the amine group of p-aminophenol being acetylated by acetic anhydride to form an amide functional group
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Date: May 22‚ 2012 Name: Cristhian Valor Organic Laboratory Pre-Lab 1. Main reaction sequence including side reaction products: 2. 3 Sentence Summary of the Experiment: The OH group on the benzene ring in salicyclic acid reacts with acetic anhydride to form an ester functional group. Thus the formation of acetylsalicyclic acid (aspirin) is referred to as an esterification reaction‚ which requires the presence of H+ (H2SO4 in our case). The technique used to purify the aspirin content
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Introduction: The purpose of this lab was to separate and purify a compound. This was achieved using techniques that allowed the extraction of the acid‚ the isolation of the neutral compound and the melting point classification of the neutral compound to test the purity of the sample. Chemical Reactions: HA + OH- A- + H2O R-COOH + OH- R-COO- + H2O Organic Acid Insoluble in H2O Conjugate base Soluble in H2O Procedure: A 0.170g sample consisting of a mixture of 0.110g
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are several purposes to the amino acid lab experiment. One reason is to help us understand the concept of acids and bases and how they interact with one another. Another purpose is to understand what occurs when placed with the existence of buffers. Lastly‚ the experiment is to help us understand the applications of titrations and how it can be used in a real world setting‚ in this case‚ the identification of an amino acid. One must understand that amino acids all have special characteristics
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Observations Figure 1 Figure 2 Figure 3 Figure 1 show the crystallisation reaction when the saturated sodium chloride solution was added to the cool reaction mixture. The salt of p-Toluenesulphonic acid started forming. Figure 2 show the wet crystal after the precipitated salt was being filtered by suction. The wet crystal was light purple in colour. Figure 3 show the end product after
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