is not in the normal blood pH‚ the person can fall sick and it might be harm to the person. The maintenance of blood pH is called acid-base homeostasis. Acid-base homeostasis is a complex synergy that involving lungs‚ kidneys and a buffer chemical in blood and blood cells. BACKGROUND A substance that has high concentration of hydrogen ion in solution is called acid and solution that has low concentration of hydrogen ion is base. Base
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The experiment consists of 5 steps—Inoculation‚ fermentation‚ cell lysis‚ inclusion body solubilization‚ and peptide purification. In the first two steps‚ E coli with Snake 6 peptides is cultivated. Ultraviolet–visible spectroscopy (UV-vis) is used to quantify bacteria through detection of their optical density (O.D.). After reaching a certain concentration in the fermentation batch‚ inclusion bodies (IBs) are extracted from these E coli by adding Bugbuster lysis solution and lysozyme. Polyacrylamide
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527 nm with a shoulder at 558 nm to 605 nm in the absorption spectrum [28]. Since there have been some reports on the complex formation between Cys and Pb2+ in an aqueous solution [30‚ 31]‚ the sensing selectivity of CA-2Pb2+ toward Cys was investigated by absorption spectroscopy. The color changes and absorption spectral study of CA-2Pb2+ (50 μM) were performed upon addition of 21various amino acids and peptide (100 μM) such as Ala‚ Arg‚ Asn‚ Asp‚ Cys‚ Gln‚ Glu‚ Gly‚ His‚ Hcy‚ Ile‚ Leu‚ Lys‚ Met‚ Phe
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gene. This can be determined by looking at the ladder and comparing our sample to it‚ to find out if we are homozygous or heterozygous. Discussion For this lab‚ DNA from our cheek cells were separated through PCR‚ and singled out through gel electrophoresis. The process of the PCR is complex and contains different components and reactions. The first main step of PCR is denaturing. The temperature at which denaturing occurs is at 94°C. This first step separates the double stranded DNA template
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AP Chemistry Name _________________________________ Period ___ Date __/__/__ 4 Reactions in Aqueous Solution Precipitate Practice #1 Write balanced molecular and detailed ionic equations. Strike out any spectator ions. 1. Solutions of lead nitrate and potassium chloride are mixed. Pb(NO3)2(aq) + 2 KCl(aq) PbCl2(s) + 2 KNO3(aq) Pb2+ + 2 NO3- + 2 K+ + 2 Cl- PbCl2(s) + 2 K+ + 2 NO3- 2. Solutions of sodium sulfate and calcium bromide are mixed. Na2SO4(aq)
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final compound which I am going to look at is 4-aminobenzenecarboxylic acid. The compound is also known as 4-Aminobenzoic acid. The molecular and structural formula of the compound is C7H7NO2 which means that the compound consists of seven carbon atoms attached to seven hydrogen atoms along with one nitrogen atom and two carbon atoms attached as a carbon-oxygen double bond. The displayed formula for the 4-aminobenzenecarboxylic acid compound is shown and it shows the formula in a ring form therefore
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Grignard reaction Abstract: In this laboratory‚ triphenylmethanol was synthesised from reacting benzophenone and bromobenzene using Grignard reaction. As the reaction was to set up to produce a Grignard reagent and then recrystallize it to obtain pure sample. The percentage yield obtained was 55% and its melting point was 161 co which is within the literature value 160-163 co. In addition to that the IR spectroscopy confirmed the molecule structure to be triphenylmethanol. Introduction: The Grignard
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Activity 1.7.2 Synthesis of Esters Purpose- the Purpose of this lab is to synthesize Esters by combining Carboxylic Acid and Alcohols. In this lab we will synthesize and then detect the odour of Esters formed. Materials- Materials that will be used in this lab are as follows:- Ethanol 7. Acetic Acid Eye Protection 11. Test tube rack Procedure- Prepare a hot-water by half filling a 500-mL beaker with water and heating it carefully on a hot plate until it comes to a gentle
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Diels-Alder cycloaddition reaction was discovered by Otto Diels and Kurt Alder and is very useful in the synthesis of polycyclic compounds. The Diels-Alder reaction can be described as: [4+2] cycloaddition- a diene with 4 π electrons + 2π electrons from the dienophile; a pericyclic concerted reaction- meaning the reaction occurs in a single step (no intermediates) and involves a cyclic redistribution of bonding electrons. In order for a Diels-Alder reaction to take place the diene must
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In order to first perform the reaction‚ 1.5 mL of 4-methylcyclohexanol was added to a pre-weighed 5mL conical vial. The conical vial was then weighed to find the actual mass of 4-methylcycloheaxanol. Then‚ 0.4mL of 85% phosphoric acid was added to the conical vial using a plastic pipet. Six drops of concentrated sulfuric acid was then added to the vial using a glass pipet. A spin vane was then added before adding the Hickman head‚ water condenser and a drying tube packed with calcium chloride to
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