Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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Stallings Period 2 January 26‚ 2013 Enzyme Catalysis Lab Report Background: Enzymes are catalyst‚ which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a reaction for the reason
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can reach a new pH level by interacting with soil. If water has a pH that is too high or too low‚ it can be life threatening to aquatic species and can corrode metal structures. After finding this out‚ I decided to test if water could reach an extreme pH just by interacting with soil around it. The question for this experiment therefore became the following. Will interaction with soil change the pH of water? I hypothesized that if water interacts with soil for three days‚ then the pH of the water will
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temperature was above 40Ëšc the enzyme did not work as efficiently‚ even with the extra energy‚ as they had become deformed. Where the enzyme does not work so well or does not even work at all the active site if the enzyme had changed. The enzyme had not died as it is not a living organism. With the shape of the active site changed the enzyme is unable to perform the "lock and key" action the enzyme is meant to do in order to catalyse a reaction. The specified enzyme is shaped to "lock" on to
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Temperature and enzyme activity Aim: To determine the effect of which the temperature of the enzyme has on the rate of the enzyme catalysed reaction. Hypothesis: The rate of reaction of an enzyme catalysed reaction will increase as the temperature of the enzyme approaches the optimum temperature. Surpassing the optimum temperature will result in a drop in enzyme activity. Materials: 6% hydrogen peroxide Liver suspension 10 test tubes 4 beakers Thermometers Measuring cylinders Test
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concept and meaning of pH 2. Provide the student experience in measuring pH a. pH testing paper 3. Test the student’s hypothesis as it related to the pH of common solutions Hypothesis The pH of the tested solutions will be in the order of the following according to a pH scale: 1. Lime juice 2. Orange juice 3. Soda 4. Iced Tea 5. Milk 6. Water 7. Soapy water Material Required To facilitate this laboratory exercise‚ the experimenter needs the following: pH strips Sample reservoirs
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of an enzyme-controlled reaction. How do these factors affect the chemical structure and properties of the enzyme. Many things can affect the rate of enzyme activity. The temperature of the enzyme‚ the pH of the solution‚ the concentration of the enzyme‚ substrate and the product. Also‚ another affector is the number of competitive and non-competitive inhibitors. As I cannot explain them all‚ I have chosen to explain the effect of temperature and also the effect of inhibitors on enzyme activity
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II: Titration with a pH Meter 1. Fill the 50-milliliter buret with a 0.25 molar NaOH solution. 2. Record volume. 3. Measure out between 20 milliliters and 40 milliliters of the unknown HCl solution. This amount must be different than the amount used in part I. 4. Record volume. 5. The amount of unknown HCl is then added to the 100-milliliter Erlenmeyer flask. 6. Insert the pH meter into the Erlenmeyer flask and record the initial pH of the acid. Remember to record the pH of the solution after
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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Properties of pH The manufacture’s claim is that Sensodyne toothpaste micro hardens tooth enamel to help protect against the effects of acid wear‚ helps stop the twinges of pain from sensitive teeth formulated with low abrasivity‚ neutral pH and gentle yet effective cleaning system. To find out if the manufactures claim on Sensodyne toothpaste is the correct level of pH. I expect the Sensodyne toothpaste to be a neutral pH level as the manufactures claim that this products pH level is neutral
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