In 1937‚ Robert Hill discovered that isolated chloroplasts can generate oxygen when they are illuminated in the presence of a suitable electron acceptor‚ even in the absence of carbon dioxide. This finding was a landmark in the study of photosynthesis because it established that the source of the electrons used in the light reactions is water. It also confirmed that the released oxygen is derived from water instead of carbon dioxide. In chloroplasts‚ the final electron acceptor is NADP+‚ which
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photosynthetic pigment is used to absorb light during the process of photosynthesis. There are five pigments usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis‚ chlorophyll a occurs in green plants while chlorophyll b
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CONTENTS S no Topic Page number 1 Introduction 1 2 Objective 4 3 Scope and limitations 5 4 Theory 6 5 Experiment 11 6 Procedure 13 7 Observations 17 8 Result 18 9 Bibliography 19 INTRODUCTION Chlorophyll is a green photosynthetic pigment found in chloroplasts of organisms like cyanobacteria‚ algae and plants. Its name is derived from the Greek words chloros‚ meaning ‘green’ and phyllon meaning ‘leaf’. First isolated
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Chapter 1:Introduction 1.1 Research Question: How does the concentration of Zinc Sulphate affect the ability of Dichlorophenolindophenol to be reduced during the light dependent reactions of photosynthesis in Spinacia oleracea? 1.2 Rationale behind research: A few years back‚ on a visit to my uncle’s farmland in Rajhistan‚ India‚ I came across a few barley farms that were clearly receiving polluted water from a zinc mine nearby. The very same crops were then sold in the market! As I recounted the
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Light Intensity Experiment: For the light intensity experiment‚ our first set of data illustrated strange correlations between the three tubes placed in front of a light source‚ so the data provided in e-campus was used. The tube that was 24 cm away from the light had the greatest decrease in absorbance over the course of 20 minutes‚ followed by the 30 cm and then the 49 cm light apparatus. The tube that was placed in the dark (wrapped in foil) remained a dark blueish-green color whereas the tubes
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Absorption of isolated chloroplasts To test absorption of isolated chloroplasts from spinach absorption readings were taken at a series of different wavelengths. Absorption Maxima of the isolated chloroplasts were observed at wavelengths of 440 and 680 with corresponding absorbance values of 0.792 and 0.589 were noted (Fig 1.). Absorption minima of the isolated chloroplasts was noted at a wavelength of 580 at an absorption of 0.291 (Fig 1.). This indicated that green light is reflected strongly
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Photosynthesis occurs in the green leaves of plants. How is photosynthesis important in the flow of carbon through an ecosystem? v \ v | | e-Text Main Menu | Textbook Table of Contents Ten C H A P T E R 1 0 ` Photosynthesis Chapter Outline CHAPTER OUTLINE Photosynthetic organisms are autotrophs. What is light? Pigments absorb light‚ which drives photosynthesis. Photosynthesis requires both photochemical and biochemical reactions to produce sugars
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objective of this experiment was to extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon adding a solvent mixture of hexane and acetone‚ the chlorophyll
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Lil Dejay C4 2/10/14 Photosynthesis: Making Energy Chloroplasts Photosynthesis is a process in which sunlight energy is used to make glucose. The site of photosynthesis is in the chloroplast – an organelle found in the leaves of green plants. The main functions of chloroplasts are to produce food (glucose) during photosynthesis‚ and to store food energy. Chloroplasts contain the pigment‚ chlorophyll. Chlorophyll absorbs most of the colors in the color spectrum‚ and reflects only green and yellow
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The Calvin Cycle Plants use energy from the sun in tiny energy factories called chloroplasts. Using chlorophyll in the process of photosynthesis‚ they convert the sun’s energy into storable form in ordered sugar molecules such as glucose. In this way‚ carbon dioxide from the air and water from the soil in a more disordered state are combined to form the more ordered sugar molecules. Carbon dioxide is captured in a cycle of reactions known as the Calvin cycle or the Calvin-Benson cycle after
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