The evolutionary relationships among cyanobacteria‚ red algae‚ brown algae‚ green algae‚ and flowering plants‚ observed from the results their photosynthetic pigment patterns using thin layer chromatography technique Introduction Endosymbiosis is a process where unicellular organisms engulf other cells resulting a life form that one or both cells are dependent on each other (Campbell et al.‚ 2008). Strong evidence suggests that different types of photosynthetic cyanobacteria underwent endosymbiosis
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Guide Table of Summary of Experiments Determination of osmotic potential Use of microscope‚ magnification‚ and measurement of cell size Observation of cells (a) animal cell: cheek cell (b) plant cell: leaf epidermis cell Enzyme activity Separation of photosynthetic pigments using paper chromatography Examining slides of transverse sections of C3 and C4 leaves Use of yeast in respiratory experiment Dissection of the mammalian digestive system Dissection of the mammalian respiratory system Examining
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light‚ the slower the reaction will take place. The Effect of Light Wavelength This experiment was conducted to observe the effect different light colors had on photosynthesis. Red‚ blue‚ green‚ white‚ and no light were the sample subjects. Spinach leaf disks were collected and placed into a beaker‚ with ten leaves under each light specified wavelength‚ with bio-carbonate to release all the excess gases. When the gases were released the disks would float. The rate at which this occurred could be
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Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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separate plant pigments using paper chromatography‚ and to measure the rate of photosynthesis in isolated chloroplasts. Because of capillary action the solvent moves up the paper causing the pigments to become visible at certain distances. The substances visible on the paper are called pigments. Chlorophyll a is the main pigment that makes up about 75% of the pigmentation in plants. Chlorophyll b makes up about 25% of the pigmentation. And carotenes and xanthophyll’s are accessory pigments that make up
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Abstract This study was undertaken to determine the relationship of different wavelengths of light and the rate of photosynthesis in spinach leafs. The rate of photosynthesis was measured every five min under light colors of white‚ green‚ red‚ blue and yellow under a light intensity of 2000 lux. The rate of photosynthesis was measured by the spinach disk method in which we replaced the air from the disks with sodium bicarbonate using a vacuum. Under photosynthesis‚ oxygen‚ a product of photosynthesis
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and thin layer chromatography of plant pigments CHEMISTRY 201L EXPT 04 PAGE 01 - 12 Noel Angelo P. Kalacas*‚ Hanna Mae Laluces‚ Ina Bianca Lanuza Department of Chemistry‚ College of Science *Corresponding author; e-mail: knight_BeNcH66@yahoo.com Abstract Chromatography is a powerful technique for separating and/or identifying the components in a mixture. There are different types of chromatography and each has its own strengths and weaknesses. In this experiment‚ pigments of the chili
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Yeasty beasties-The effect of reaction rate on yeast fermentation 22. Suck It Up: Capillary Action of Water in Plants- dye in water with white carnations 23. What Color Are the Leaves Really Turning? colors of fall by separating plant pigments with paper chromatography 24. Attack of the Killer Cabbage Clones. How do you clone a plant 25. Can Water Plants Be Used to Determine Water Quality? 26. Are There Bugs Under Your Feet?- numbering the microorganisms in your own yard http://www.all-science-fair-projects
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Chromatography Lab Questions Pre-Lab Questions 1. A pencil is used rather than a pen to mark the “starting line” in step two because a pen or marker while be used to place a small dot of ink in the center of the X that is made with a pencil. 2. Capillary action is filling of pores in a solid with a liquid. Capillary action is caused by spotting or absorption and is allowed to seep or flow through. 3. The type of pen that will probably give better separation in this paper chromatography
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and Isomerization of Lycopene from Tomato Paste Goal / Purpose To extract the carotenoid pigments from canned tomato paste and separate them by column chromatography to obtain a solution that contains lycopene. Then record the ultraviolet-visible spectrum of this solution and analyze it for evidence of isomerization. Calculation List of Materials 4.0g tomato paste screw cap vial Chromatography column 25ml buret 50ml flask ring stamd and clamp 50% mixture of acetone and low boiling
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