Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
Premium Photosynthesis Color Green
experiment we used paper chromatography to determine the amount of chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta-carotene in a tube of blended leaf extract. We recorded our findings on the table labeled table 1. Table 1 shows the transmittance at each wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used; with the help of acetone we saw the spectrum and the differences of the different pigments. Percentage transmittance
Premium Chlorophyll Digestion Pigment
Journal of the American Chemical Society 63: 877-879. Sterling‚ T.‚ Ph.D. 2004. Transpiration-Water Movement Through Plants. Zafaralla‚ M.T.‚ et.al. 2007. Explorations in Plant Science. pp. 63-66. Henderson State University. Separation of Pigments by Paper Chromatography. Retrieved from: http://www.hsu.edu/pictures.aspx?id=1653
Premium Plant Photosynthesis Pigment
Spinach Leaves The goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a‚ chlorophyll b‚ carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the data was graphed it clearly showed the higher rate of red light absorption over green light. These
Premium Photosynthesis Electromagnetic radiation Chromatography
Question 1: You have carried out an investigation into the pigments that make up chlorophyll. Use the chromatogram you obtained when carrying out this investigation. Clearly explain the separation technique of paper chromatography in terms of partition. Calculate the Rf value of one of the constituent pigments and compare it to the book value. A paper chromatography was used to separate the pigments that make up chlorophyll to analyse‚ identify‚ and quantify the components‚ using paper (stationary
Premium Chromatography Chemical polarity Atom
AP Biology Lab Four: Plant Pigments and Photosynthesis Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene‚ the most abundant carotene in plants‚ is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found
Premium
Scope and limitations 5 4 Theory 6 5 Experiment 11 6 Procedure 13 7 Observations 17 8 Result 18 9 Bibliography 19 INTRODUCTION Chlorophyll is a green photosynthetic pigment found in chloroplasts of organisms like cyanobacteria‚ algae and plants. Its name is derived from the Greek words chloros‚ meaning ‘green’ and phyllon meaning ‘leaf’. First isolated
Free Photosynthesis Chlorophyll
Bioenergetics Photosynthesis & Respiration Laboratory Report Exercise 6 PBIO101 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Gina Dedeles Minda Dimaano-Kho Group 5 Felicita‚ Haniel Paulo‚ Gisselle Mildred V. Aniseta‚ Carmelus*Absent but present
Free Carbon dioxide Photosynthesis Oxygen
CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
Premium High performance liquid chromatography Chromatography Analytical chemistry
to evaluate the process and the significance of photosynthesis‚ define the light compensation point‚ observe the effect of light intensity and wavelength on the photosynthetic rate of a Spinach leaf‚ and identify the pigments found in the chloroplasts of spinach leaves by using a Thin Layer Chromatography
Premium Photosynthesis Oxygen Carbon dioxide