this experiment was to successfully ligate RFP into P1 and transform E. coli with P1-RFP plasmid‚ which contains the P1 promoter‚ RBS‚ and RFP gene. We tested several different ligation conditions and assessed how they each affected ligation efficiency. The two variables that we tested were: molar ratio of insert (RFP): vector (P1 plasmid)‚ as well as the effect of removing the stuffer from the digested plasmid. We predicted that the cells transformed without stuffer would have higher ligation efficiency
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When Chemicals React! Mr. Bell’s honors level chemistry class conducted an experiment during their lab demonstrations‚ this consisted of elements such as phosphorus and calcium chloride in their experiment. This along with another hydrogen based sunstance produced‚ what looked like a pinkish-looking substance inside of their flasks that were at their lab stations. Sophmore Kelly Caudel said‚ “ I actualley enjoy doing the experiments in this class‚ because it gives us a chance to get away from
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Alan Shawn Feinstein Graduate School Global Economics Environment Prof. McDonaldAmini White Paper Outline Internet Restriction Xiaonan Jiang Introduction The first amendment in the constitution of the United State of America states the freedom of religion‚ press and expression would be cornerstone for the democracy and the base of all human rights. Nowadays‚ freedoms of the speech are not limited only towards publication of articles‚ criticism
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The Real Monster of the Midway Serial Killers are nothing new to today’s society. Jeffrey Dahmer‚ John Wayne Gacy and Ted Bundy shocked our nation with their violent‚ brutal‚ and at times unbelievable crimes‚ although none of them were as notorious as Herman Webster Mudgett better known as Dr. H.H. Holmes. A man that carried himself as a smart‚ intellectual member of society‚ but in reality he was a smooth talking conman and very disturbed individual. A man who designed and built a building with
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Biophysical Ecology Abstract: There are many patterns that can be observed throughout our environment. In this experiment‚ the temperatures of organisms in a biophysical environment were analyzed to see if a pattern could be recognized that was related to the behavior of an ectotherm. An ectotherm is an organism that relies on the environment to regulate its body temperature. Organisms such as Pseudemys (turtles) and Lacertilia (lizards) are examples or ectotherms. After arriving at Maxcy Gregg
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Name: Nikia Martinez Class: Biology 240L L3-1201 Assignment: Electrocardiography Lab Report Due: April 3rd 2012 Professor: Dr. B. Schoffstall Introduction In a normal human being the heart correctly functions by the blood first entering through the right atrium from the superior and inferior vena cava. This blood flow continues through the right atrioventricular valve into the right ventricle. The right ventricle contracts forcing the pulmonary valve to open leading blood flow through the pulmonary
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Mustaqim Ahmed Period 4 September 18‚ 2013 Analysis of Food Dyes in Beverages Purpose: To determine the concentration of dye in a sports drink using spectroscopy and graphical analysis. We will be using beers law to find the unknown concentration of dye in a sports drink. Materials: Beakers‚ 50-mL‚ 2-3 ; Test tubes (13 x 100)mm 3-8 ; FD&C Blue 1 stock solution‚ 50-mL ; Lens tissues ; Pipets‚ serological‚ 10-mL ; Sample of consumer sports drink‚ 10-mL ; Spectrophotometer ; Test tube rack Procedure:
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Homeostasis Lab: The Effects of Exercise on Homeostasis Purpose: To discover the effect that various levels of exercise have on specific body parameters. Hypothesis: If a subject walks up and down a flight of stairs for eight minutes‚ then they start to sweat more‚ their heart rate‚ body temperature‚ and breathing rate will increase‚ and there will be a change in their skin color on their arms and face‚ because the body will be trying to maintain homeostasis. Materials: You will
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Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The
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potential to damage crops extensively‚ and thus‚ damage society as well through shortage of food or even famine. In addition‚ in countries that depend on the production of agricultural goods like Canada‚ the effects of acid rain can be tremendous. In this lab‚ we have chosen to examine the effects of acidic and basic environments on radish plants (Raphanus sativus)‚ by implementing varying pH levels of buffer solution into the soil. We have allotted a total of four days (Tuesday‚ April 10‚ 2012- Friday April
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