optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin‚ which is a translucent‚ colourless‚ brittle solid substance found in the collagen inside an animals’ connective tissues. In my experiments it is going to be in the form of a single‚ thin layer‚ used on the surface of photographic film. It is useful in photography because it acts as protein glue‚ sticking the silver halide crystals to the surface of the plastic film. I am using it in this
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liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) . Chemical reactions occur when two or more molecules interact and the molecules change. Bonds between atoms are broken and recreated to make new molecules. Enzymes are large proteins that speed up the rate of a chemical reaction by acting as a catalyst and remain unchanged‚they are natural biochemical catalyst. Certain catalysts work
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Indicates acute inflammation of glomeruli hematuria (including red cell casts) mild to moderate proteinuria oliguria‚ hypertension and mild edema 2.Nephrotic syndrome Indicates excessive permeability of the filtration membrane to plasma proteins. Clinical manifestations of renal diseases (contd..) heavy proteinuria (adult more than 3.5 gm/day) hypoalbuminemia (less than 3.0 gm/dL) severe generalized edema Hyperlipidemia and lipiduria 3.Asymptomatic proteinuria
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CELL INJURY: CAUSES OF CELL INJURY‚ MECHANISMS OF REVERSIBLE AND IRREVERSIBLE CELL INJURY. CELL INJURY. Causes of cell injury range from gross mechanical external causes to mild endogenous causes as genetic lack of enzymes etc. Virtually all forms of tissue injuries start with molecular or structural alterations in cells. Under normal conditions‚ the cells are in: ❖ homeostastatic „steady“ state Normal cell is confined to relatively narrow range of functions and structure by
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Biological Molecules Experiment-Specific Questions Experiment 1: Monosaccharide Test 1. Fill in the table below with the results from the monosaccharide test experiment‚ and your conclusions based on those results. Results Monosaccharide Test Solution Initial Color Color with Benedict’s Solution Color After Heating Monosaccharide? glucose solution clear blue orange yes water clear blue blue no sucrose solution clear blue
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Dialysis Experiment: Diffusion of Polar and Non-Polar Substances Introduction It was hypothesized that if the solution is polar‚ then it will diffuse through the dialysis bag. All five solutions of starch‚ albumin‚ sodium sulfate‚ sodium chloride‚ and glucose were placed inside of a dialysis bag‚ which was then placed in a culture dish filled with distilled water. After waiting 60 minutes‚ for the solutions to escape the bag‚ the distilled water was tested for all five solutions through individual
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Enzymes are large globular proteins which act as biological catalysts. They increase the rate of reaction without being used up by themselves. They are found in and out of cells and lower the activation energy of a reaction. Amylase is the enzyme which catalyses starch hydrolysis. Alpha amylase and beta amylase are two types of amylase enzymes. The amylase which is the most commonly found in the human body is the alpha amylase. Beta amylase is mainly found in bacteria‚ fungi and plants. Amylase breakdown
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Protein Quantitation using the Bradford assay and Spectrophotometry Protein quantitation is a study which is used to determine protein concentration in an unknown sample by comparing its assay response to one which is already known. This has uses in a broad range of research topics which include protein analysis. Two bovine serum albumin (BSA) dilutions of unknown concentrations using the Bradford assay method of protein quantification were used. BSA is a fatty acid carrier protein in the blood;
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speculations‚ more or less well- founded‚ on their physical properties (denaturation‚ hypochromic- ity‚ etc.)‚ on the transfer of biological information from deoxy- ribonucleic acid to ribonucleic acid‚ and on the role of the latter in directing the synthesis of specific proteins. They form the basis of present explanations of the manner in which the amino acids are activated before being assembled to make a protein; they are being invoked incessantly in attempts to unravel the nucleotide
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(eg‚ asthma and rhinoconjuctivitis)‚ gastrointestinal tract (eg‚ vomiting‚ diarrhea‚ and colic)‚ or systemic anaphylactic shock. Cow’s milk contains more than 25 different proteins that are among the best characterized food allergens. Among these proteins only whey proteins alpha-lactalbumin‚ beta-lactoglobulin‚ bovine serum albumin (BSA)‚ and lactoferrin‚ as well as the four caseins‚ have been identified as allergens. A study evaluated cow’s milk allergens
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