highly pigmented protein that imparts the characteristic color to certain blue-green algae. It also facilitates a reaction necessary to the survival of this species; we can follow the kinetics of this reaction by measuring the conversion of Substance X to Substance Y at various times during purification.” Techniques and methodologies for extracting the proteins and purifying Enzyme X are as follows: The blue-green tinted proteins in Enzyme X give a distinct color to algae. The proteins are contained
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Environmental Report on the Value of Trees Trees play a significant part in our community and to our local wildlife as they improve and provide a number of important environmental benefits. They provide better air quality for us by filtering the airborne pollutants and by removing the atmospheric carbon dioxide from the atmosphere and places with cleaner air (places with more trees) extend people’s life force by months or even years. This impacts the organisms by contributing to the carbon cycle
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PHYSIOLOGIC VALUE OF FOOD Organic compounds * Protein * Lipids * Carbohydrates * Vitamins Inorganic elements * Water * Minerals Vit & min * Act as a catalysts which prompt the 3 major nutrients –carbohydrates ‚ protein‚fats METABOLISM * “Metabolismos”-to change or alter * Chemical process of transforming foods into complex tissue elements and of transforming complex body substance into simpler ones‚ along with the production of heat energy * Is the totality of the
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Biochemistry of Proteins; Isolation of Ovalbumin and Enumeration of thiol groups Abstract Thiol groups are important to protein folding and forming disulphide bonds that are essential to protein structure. Determining the number of thiol groups in a protein is important in determining the tertiary structure of the protein. The ovalbumin is the experiment was purified from egg white using centrifugation and ammonium sulphate precipitation and then the thiol groups identified using DTNB and spectroscopy
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Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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April 22‚ 2015 Does protein help to build muscle? The latest craze is about protein and how it affects building muscles and building muscle strength. Today‚ there are many athletes that use it as a supplement. There are many protein supplements available that are claiming to help people who work out to build muscle faster. Protein is a structure within our bodies that consists of amino acids. The cells in our bodies need this to function properly. If we did not have protein‚ our body’s functions
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INTRODUCTION Shaving gel is a viscous‚ clear gel preparation which is applied before shaving in order to soften the beard‚ lubricate the passage of razor over the face and moisturize the skin. Gels have cross-linked system which exhibits no flow in steady state. Desired characteristics of a Shaving Gel: * It should be clear and transparent. * It should have thick consistency but flowable. * It should lather easily when applied with brush. * It should give clean‚ fresh and moisturized
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Protein Article Research John Doe SCI 241 May 17‚ 2012 Jane Doe Protein Article Research This class has been very informative during the past few weeks. We have learned how to count calories‚ read nutrition labels‚ use a tracker to monitor our daily intake‚ carbohydrates‚ and learned about proteins and their effects. According to the readings in the text and the article proteins are the building blocks in the body. I learned that protein is found in all tissues‚ cells and organs of the body
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assays to detect the presence and absence of certain antigens or antibodies and also to quantify them when necessary. Quantification can be done in a range of microgram (µg) to nanogram (ng). The ELISA procedure takes advantage of the fact that most proteins will bind firmly to the surface of different kinds of plastic (polystyrene or polyvinyl chloride)‚ usually by hydrophobic interaction. The steps of the ELISA procedure are simple to perform. There are mainly FOUR phases in ELISA: 1. Coating
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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