Synthesis and Purification of Acetylsalicylic Acid (ASA or Aspirin) Background Salicylic acid is a phenol as well as a carboxylic acid. It can therefore undergo two different types of esterification reactions‚ creating an ester either with the hydroxyl or with the acid. In the presence of acetic anhydride‚ acetylsalicylic acid (aspirin or ASA) is formed. Correspondingly‚ an excess of methanol will form methyl salicylate‚ which is also an analgesic. In this experiment‚ we shall use
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amount of protein‚ and percent yield and an increasing trend for specific activity and fold purification. These trends come about naturally when performing multiple purification steps. To determine the success of each purification step‚ the more important factors to look at are the total activity units‚ total protein amount‚ and percent yield. Looking at our purification table (table 7) our purifications were not very successful. While our total protein followed the normal decreasing trend‚ as
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Acids‚ Bases‚ and pH Lab In this lab the testing of whether or not a substance was an acid or a base occurred. Each substance was tested with the indicators red litmus paper‚ blue litmus paper‚ pH paper‚ phenolthalein‚ bromthymol blue‚ and phenol red. While the substances were tested the group noticed that the substances tested with the red and blue litmus paper‚ the phenolthatein‚ bronthmol blue were the easiest to interpret. The color changes that occurred when this indicator was put into a substance
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Determining Red 40 Concentrations Using Absorption with Beer’s Law Introduction I like color and enjoyed learning about wavelengths and the spectrum of light‚ so I considered incorporating something related to that into my Internal Assessment. We also had just used concentrations in our Group 4 Project‚ so when I found an experiment that dealt with both of these I thought it was a great idea. This experiment is not completely original; the basic concept has been used multiple times. It uses Beer’s
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To purify the protein in the cell lysate from lab 1 through nickel affinity chromatography. Protein purification should result in only one type of protein ideally‚ which is the protein of interest‚ wt-DHFR and mut-DHFR in this case. A pure protein allows for further analysis on the protein to be conducted‚ such as its concentration (Bradford assay)‚ its molecular weight‚ and its biological activity. 2. Overview of experiments Buffer Preparation Add the liquid sodium phosphate‚ solid sodium chloride
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Exercise 10: Acid-Base Balance: Activity 2: Rebreathing Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. In cases of acidosis‚ the pH of the blood is You correctly answered: c. less than 7.35. 2. Carbon dioxide and water form You correctly answered: a. carbonic acid (a weak acid). 3. Which of the following is true of respiratory acidosis? You correctly answered: c. The amount of carbon dioxide in the blood is greater than normal. 4. Rebreathing You correctly
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409 Lab 40: Calorimetry Calorimetry is the measurement of the quantity of heat exchanged during chemical reactions or physical changes. For example‚ if the energy from an exothermic chemical reaction is absorbed in a container of water‚ the change in temperature of the water provides a measure of the amount of heat added. Calorimetry involves the use of a calorimeter. In this activity you will learn how the energy change in a physical change can be measured using a calorimeter. •
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Purity and Purification of Solids Melting Point Lab Introduction: The point of this lab was to determine the eutectic point for the naphthalene biphenyl mixture‚ as well as determining the melting point of an unknown substance by comparing it with two known samples. Melting point is a temperature in which a substance changes from solid state to liquid state. Melting points are used to determine whether the given substance is pure or not. Substances that melt sharply‚ less than 1-2°C indicates
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification Maintain the PCR volume of 50µL-100 µL. Pipet the elution buffer in the center of the PureLink® Spin Column (PSC) and perform an incubation. Add B2 to PCR reaction. Add this sample to the PSC. Centrifuge the PSC. Replace the PSC into the wash
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