The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
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For known amounts of reactants‚ theoretical amounts of product can be calculated in a chemical process. These calculated products are determined using the limiting reagent‚ (the substance that runs out first which stops the reaction) and are called the theoretical yield. The theoretical yield is the amount of product that should be acquired during the experiment if all aspects go perfectly. However‚ due to experimental errors‚ very few labs do so. These results are called actual yield. The percent
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necessary to dilute the Sodium Hydroxide so that it is an easier concentration to titrate. Using a 25cm3 pipette‚ washed out with some of the NaOH solution‚ transfer 25cm3 of the solution to a 250 cm3 volumetric flask‚ and fill to the line with distilled water. The alkali will now have a concentration of 0.1 mol dm-3. • In the titration‚ the NaOH will be in the burette‚ and will be titrated into HCl in a conical flask: NaOH (aq) + HCl (aq) NaCl (aq) + H2O (l) • The reaction is 1:1 between NaOH and
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Sodium is a mineral that is present only in small quantities in most natural foods‚ but salt is added‚ often in large amounts‚ in food processing and by cooks to enhance flavor. Sodium is the predominant ion in extra cellular fluid. Sodium (Na) is the predominant cation in extra cellular fluid and its concentration is under tight homeostatic control. Excess dietary sodium is excreted in the urine. The kidney very efficiently reabsorbs the mineral when intakes are low or losses are excessive. Sodium
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Atausch July 28‚ 2014 Bio 102 Lab Final Exam: Acids and Enzymatic Reaction Purpose: To examine how an acidic fluid will modify the outcome of an enzymatic reaction. Introduction: Enzymes are “specialized proteins made by living cells that serve as biological catalysts.” (eScience Labs‚ LLC‚ 2013) These enzymes “allow certain industrial processes to be carried out at normal temperatures and pressures‚ thereby reducing the amount of energy and expensive equipment needed.”(BBC‚ 2012) Enzymatic
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affecting reaction ratesProcedure A: Effect of ConcentrationAim: To investigate how the concentration of a reactant affects the rate of reaction. Hypothesis: The more concentrated the reactant‚ the faster the rate of reaction. Materials: 2M hydrochloric acid solution‚ magnesium wire‚ distilled water‚ 4 test-tubes‚ measuringcylinder. Procedure:1)10mL 2M hydrochloric acid solution is poured into a test-tube using a measuring cylinder. 2)A 3cm length of magnesium wire is added to the acid‚ and the
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Experiment 7 INVESTIGATING STOICHIOMETRY WITH SODIUM SALTS OF CARBONIC ACID Introduction In this experiment we are going to get a better understanding of chemical stoichiometry. We are going to be reacting sodium bicarbonate (NaHCO3) and sodium carbonate (Na2CO3) with hydrochloric acid (HCl). To start off the mass of two unknown substances (being the sodium bicarbonate and sodium carbonate) will be taken. We will need to construct balanced equations for both of the reactants with the HCl and
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the reaction will occur) has been proven correct and it proves the theory right. From the results‚ we can see this‚ as the iced water (10 degrees) had the slowest rate of reaction at 1.10 minutes. Then the room temperature water (19 degrees) was tested and had a slightly better rate of reaction at 1.09 minutes. Then last of all‚ we tested the boiling water (63 degrees) which had a large improvement from the last two with a rate of reaction of 43.5 seconds. This showing development in the rate of reaction
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2. An aqueous solution of KCl is colorless‚ KMnO4 is purple‚ and K2Cr2O7 is orange. What color would you expect of an aqueous solution of Na2Cr2O7? Explain. Orange. 3. Explain why hexane will dissolve benzene but will not dissolve sodium chloride. Sodium chloride is an ionic compound. 4. Some drinks like tea are consumed hot or cold‚ whereas others like Coca-Cola are consumed only cold. Why? For taste reasons‚ these drinks can be drunk if the user likes It but having coca cola like tea
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Analysis of Soda Ash and Volumetric Analysis of a Carbonate-Bicarbonate Mixture Buti‚ Mary Daphne A. Department of Chemical Engineering‚ Faculty of Engineering University of Santo Tomas España‚ Manila Abstract Sodium carbonate (Na2CO3)‚ commonly known by trade name soda ash‚ is a white‚ anhydrous‚ powdered or granular material that is an essential raw material used in the manufacturing of glass‚ detergents‚ chemicals‚ and other industrial products. In this experiment‚ the percent alkalinity
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