forming reduced copper in alkaline solutions. This is called a "Biuret" chromophore. The second is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate) by tyrosine and tryptophan residues. The reduced Folin-Ciocalteu reagent is blue and therefore is detectable with a spectrophotometer in the range of 750 nm. Using the Folin-Ciocalteu reagent to detect reduced copper makes the assay nearly 100 times more sensitive than the Biuret reaction alone. The BCA Assay: The BCA
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pentahydrate CuSo4.5H2o AR Sinopharm Chemical Reagent Co.Ltd Sodium hydroxide NaOH AR Sinopharm Chemical Reagent Co.Ltd Potassium Sodium Tartrate Tertrahy drat C4H4O6KN4 .4H2O AR Sinopharm Chemical Reagent Co.Ltd Silver Nitrate AgNO3 AR Sinopharm Chemical Reagent Co.Ltd Trisodium Citrate dihydrate C6H5Na3O7.2H2O AR Sinopharm Chemical Reagent Co.Ltd Hydrogen Peroxide H2O2 AR Sinopharm Chemical Reagent Co.Ltd Sodium borohydride NaBH4 AR Sinopharm Chemical Reagent
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is Correct answer: e. starch and carbohydrate. You have not answered this question. 2. Which of the following is true of enzymes? Correct answer: c. Their activity can be affected by temperature and pH. You have not answered this question. 3. The reagent IKI tests for the presence of Correct answer: a. starch. You have not answered this question. 4. Which of the following is not true of controls? Correct answer: d. A negative result with a positive control is required to validate the test You have
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CHEMISTRY PROJECT 2013/2014 NAME: OSANYINTUYI MOTUNRAYO ESTHER DEPARTMENT: COMPUTER SCIENCE FACULTY COMPUTER SCIENCE AND MATHEMATICS PROJECT TITLE DETERMIATION OF TOTAL HARDNESS IN ILARA COMMUNITY DRINKING WATER TABLE OF CONTENT. ABSTRACT INTRODUCTION MOTIVATION LITERATURE REVIEW PROBLEM STATEMENT EXPERIMENT RESEARCH QUESTIONS DISCUSSION CONCLUSION ABSTRACT This project was done to determine the total hardness of ilara mokin water to show the usefulness
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1. Title: The Process of Determining the Unknown Bacteria #9 Rachel Judecki July 5‚ 2011 2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification‚ the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB)‚ TSI (Triple Sugar Iron agar)‚ Phenol
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directly proportional to the protein concentration‚ according to the Beer-Lambert law. For this test‚ a specific reagent is needed‚ in this case‚ the biuret reagent. The alkaline solution is made of potassium hydroxide‚ a strong base. A compound of hydrated copper sulfate and potassium sodium tartrate is dissolved in this solution‚ turning blue. In the presence of proteins‚ the reagent turns violet‚ but turns from blue to pink in the presence of short-chain polypeptides‚ or basic amino acids. This
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9-48 MICROBIOLOGICAL EXAMINATION (9000) 9221 MULTIPLE-TUBE FERMENTATION TECHNIQUE FOR MEMBERS OF THE COLIFORM GROUP* 9221 A. Introduction The coliform group consists of several genera of bacteria belonging to the family Enterobacteriaceae. The historical definition of this group has been based on the method used for detection (lactose fermentation) rather than on the tenets of systematic bacteriology. Accordingly‚ when the fermentation technique is used‚ this group is defined as all facultative
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can undergo air oxidation requiring that the procedure be performed with minimal delay. Reagents: | | starch solution | 1.5 M H2SO4 | | potassium bromide | potassium bromate | | potassium iodide | potassium iodate | POTASSIUM BROMATE EXPERIMENTAL PROCEDURE (this is an alternative procedure) Preparation of Standard 0.015 M Potassium Bromate Weigh approximately 2.6 g of dried and cooled reagent grade KBrO3 to the nearest 0.1 mg. Transfer quantitatively to a 1000 mL volumetric flask
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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Topic 8a – Carbonyl compounds Revision Notes 1. Introduction • Aldehydes and ketones are carbonyl compounds • They contain the carbonyl group C=O • The functional group in aldehydes is –CHO on the end of a chain e.g. ethanal CH3CHO • The functional group in ketones is C=O not at the end of a chain e.g. propanone CH3COCH3 2. AS Recap • Primary alcohols are oxidised by acidified potassium dichromate. An aldehyde is produced first and this can be further oxidised
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