University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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filling it with water and adding ice cubes until it showed 10°C on a thermometer. Adding two test tubes‚ one with the solution and one with the catalase‚ while still in their test tubes‚ they were allowed a 10 minutes for the catalase and solution to reach 10°C. Following the wait time‚ each test tube had a seperate thermometer placed in them to avoid starting the reaction early due to cross contamination and to check if they had reached 10°C. OR The tub was prepared by filling with water and adding boiling
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Introduction: Crayfish also known as Procambarus clariia are classified as a decapod crustaceans related to lobsters‚ crabs‚ and shrimp. They breathe through the gills as they are aquatic animals but they can survive for short time outside the water. They have a hard‚ calcified exoskeleton that must be shed as they grow. In front of the first abdominal segment locates crayfish heart in the dorsal portion of cephalothorax. Crayfish are poikilothermic as their internal temperature is proportional
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Mutations of E. coli Lab Report Advanced Biology Deducing Mutations of the Lac Operon of E. coli Abstract: In this lab we determined a possible mutation in unique bacterial strains of E. coli by observing the proper responses of wild type E. coli Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes‚ each containing a different substance (lactose‚ glucose‚ water and lactose and glucose). After a warm water bath‚ Sarkosyl detergent
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Meiosis and Genetic Diversity in Sordaria 979554296 Biology 110 Lab Introduction: In Israel there exists multiple spots in the mountains called Evolution Canyons‚ which are all located between a southern facing slope (SFS) and a northern facing slope (NFS). What’s particularly interesting about these locations is that despite the two slopes being on opposite sides of a small canyon‚ they exhibit extremely contrasting conditions. The SFS receives multiple times the UV radiation from the sun
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Lab report for Experiment #2: Extraction Your Name: Name of TA: Lab Partner’s Name: Lab Section: Title: Experiment #2: Extraction Purpose: What is the purpose of this lab? In your OWN words! Observations: Weighed out 3.2568 grams of chemical mixture that was yellow in color. Dissolved dry chemicals in 38 ml CH2Cl2 with gentle heating. Poured the yellow solution into sep funnel. Added 10 ml CH2Cl2 to flask to rinse‚ poured solution into sep funnel. Added 15 ml 3 M
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column above it‚ we can calculate the hydrostatic pressure by: The sumary of the theory is the force on any flat surface is the average pressure acting on the submerged surface multiplied by the area of the submerged surface. F = ρgXA Where: ρ = water density g = acceleration due to gravity X = vertical distance from free surface to centroid of A We know that the magnitude of the distributes force F‚ which may be considered as a small series of small forces spread over the submerged surface
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Objectives: 1. To identify the relative densities of some common liquids and solids. 2. To determine the densities of water‚ an unknown liquid‚ a rubber stopper‚ and an unknown rectangular solid. 3. To determine the thickness of a piece of aluminum foil using the density concept. 4. To practice proficiency in performing the following experimental procedures: pippeting a liquid‚ weighing by difference‚ and determining a volume by displacement. Apparatus: Tall glass cylinder‚ glass marble‚ rubber
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Physics Lab report Aammar Paracha Section MX Feb 6th 2015 OPTICS Abstract: This experiment revolves around using light rays and lenses. The experiment also discusses different properties of light rays. This experiment uses different lenses and measurements to produce images and observes different techniques to obtain the image position. Question and Answers: 1. Use your data to verify the Law of Reflection and then use Snell’s Law to calculate
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Lab Report Procedure 6.1: Perform Benedict’s test for reducing sugars. Introduction: Benedict’s tests allows for the detection of the presence of reducing sugars. All monosaccharides are reducing sugars since all of them have active carbonyl group. Some disaccharides that are exposed to a carbonyl group are also reducing sugars but less reactive than monosaccharides. By mixing the sugar solution with Benedict’s solution and heating them‚ a redox reaction will occur. The copper (II) sulphate
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