Catalase Lab Report
EFFECT OF TEMPERATURE ON THE RATE OF CATALASE REACTION IN HYDROGEN PEROXIDE
INTRODUCTION
Enzymes function similarly to a lock a key mechanism where only one specific key will work (U and Fischer, 1994). An enzyme is a macromolecule that acts to increase the rate of either a substrate breaking down into two or more products, or where two substrates join up to form one product (Dundon, 2018). These enzymes perform this by lowering the activation energy required for the reaction to occur under normal conditions. For the enzymes to lower the activation energy they can either orientate two or more substrates so that they are more likely to form the interactions required than if they were in free space, or they can break a substrate into multiple …show more content…
The results will further link into a conjecture that can be formulated regarding temperature and rate of reaction. To keep the testing fair and consistent, several constants were introduced prior to testing. One key point to focus on is that the source of the catalase remains constant, to prevent results where one catalase from one liver may not be as effective as another. Further regarding the catalase, the size of the pieces used needs to remain consistent at 1cm3 as this helps keep the surface area constant removing chances of greater reaction rates. Another deciding factor is keeping the time allowed for the foam development to remain at 5 seconds throughout all testing, to prevent the event of overflow, or the gradual decrease in rate of reaction over a long timeframe. Furthermore, providing the volume of hydrogen peroxide remains at 3mm and the size of the test tubes is contained, a fair test of the foam formation will …show more content…
One set was then filled with 3mL of hydrogen peroxide solution each. The height of the solution was measured from the test tubes bases and recorded, ensuring it was measured to the bottom of the meniscus.
The liver was then cut into six 1cm3 pieces using a surgical scalpel and one piece was placed into each of the empty test tubes. A tub was prepared for testing by filling it with water and adding ice cubes until it showed 10°C on a thermometer. Adding two test tubes, one with the solution and one with the catalase, while still in their test tubes, they were allowed a 10 minutes for the catalase and solution to reach 10°C. Following the wait time, each test tube had a seperate thermometer placed in them to avoid starting the reaction early due to cross contamination and to check if they had reached 10°C.
OR
The tub was prepared by filling with water and adding boiling water from a kettle until it reached the desired temperature. Two test tubes; one with catalase and one with solution, were placed into the tub and left until they reached the desired temperature on seperate thermometers to mitigate chances of
INTRODUCTION
Enzymes function similarly to a lock a key mechanism where only one specific key will work (U and Fischer, 1994). An enzyme is a macromolecule that acts to increase the rate of either a substrate breaking down into two or more products, or where two substrates join up to form one product (Dundon, 2018). These enzymes perform this by lowering the activation energy required for the reaction to occur under normal conditions. For the enzymes to lower the activation energy they can either orientate two or more substrates so that they are more likely to form the interactions required than if they were in free space, or they can break a substrate into multiple …show more content…
The results will further link into a conjecture that can be formulated regarding temperature and rate of reaction. To keep the testing fair and consistent, several constants were introduced prior to testing. One key point to focus on is that the source of the catalase remains constant, to prevent results where one catalase from one liver may not be as effective as another. Further regarding the catalase, the size of the pieces used needs to remain consistent at 1cm3 as this helps keep the surface area constant removing chances of greater reaction rates. Another deciding factor is keeping the time allowed for the foam development to remain at 5 seconds throughout all testing, to prevent the event of overflow, or the gradual decrease in rate of reaction over a long timeframe. Furthermore, providing the volume of hydrogen peroxide remains at 3mm and the size of the test tubes is contained, a fair test of the foam formation will …show more content…
One set was then filled with 3mL of hydrogen peroxide solution each. The height of the solution was measured from the test tubes bases and recorded, ensuring it was measured to the bottom of the meniscus.
The liver was then cut into six 1cm3 pieces using a surgical scalpel and one piece was placed into each of the empty test tubes. A tub was prepared for testing by filling it with water and adding ice cubes until it showed 10°C on a thermometer. Adding two test tubes, one with the solution and one with the catalase, while still in their test tubes, they were allowed a 10 minutes for the catalase and solution to reach 10°C. Following the wait time, each test tube had a seperate thermometer placed in them to avoid starting the reaction early due to cross contamination and to check if they had reached 10°C.
OR
The tub was prepared by filling with water and adding boiling water from a kettle until it reached the desired temperature. Two test tubes; one with catalase and one with solution, were placed into the tub and left until they reached the desired temperature on seperate thermometers to mitigate chances of