Abstract This experiment aimed to study the effect of various denaturants on albumin and casein protein extracts through viscosity measurements. 5 mL samples of native and denatured protein solutions were prepared‚ using -mercaptoethanol‚ urea and SDS as denaturants for albumin‚ and NaOH‚ NaCL‚ HCL‚ -mercaptoethanol‚ urea and SDS for casein. 5 mL blank solutions for each denaturant used were also prepared. The viscosity of the solutions were determined using Ostwald viscometer. ________
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is 60.07 and is to great to pass through the 20MWCO membrane. • Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? My prediction was correct in this experiment. Knowing that glucose is a monosaccharide‚ it is of size to diffuse through a 200MWCO membrane. Albumin being a protein is dense with 607 amino acids and an average molecular weight of 135g/mole is not going to diffuse through the 200MWCO
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of the experiment correlated with my prediction. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? As expected‚ glucose‚ with a molecular weight of approximately 180 g/mol‚ readily diffused across the 200 MWCO membrane; whereas‚ albumin did not diffuse across the 200 MWCO. Albumin is a protein composed of 67 amino acids and its molecular weight is significantly greater than 200 g/mol‚ accordingly
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2.4. Animals One hundred twelve adult male Wistar rats (Pasteur Institute‚ Tehran‚ Iran) weighing 220-250 g were housed in groups of four per cage in a controlled environment (temperature 23±1°C‚ 12h light/dark cycle) with free access to food. Rats were habituated to the new environment for seven days before the test. All experiments were performed in accordance with the guide for the care and use of laboratory animals (IR.SBMU.nrc.REC.1390.20) approved by the Research and Ethics Committee of the
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the release of hormones‚ glucagon and adrenalin‚ to stimulate the release of fatty acids in adipocyte tissues by activating the enzyme traicyclglycerol lipase. This enzyme breaks down the triacylglycerols into fatty acids and glycerol‚ while the serum albumin transports the fatty acids through the bloodstream to deliver them to the target tissue‚ and ultimately into the muscle cells where the fatty acids can be oxidised. The process of the oxidation of fatty acids‚ β-oxidation will be discussed in depth
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Identifying Biological Molecules with Colorimetric Chemical Tests Objectives Perform a series of accurate tests on biological molecules to detect the presence of carbohydrates and proteins‚ as well as the action of an enzyme on specific molecules. Introduction Unknown biological molecules can be correctly identified using various tests‚ including colorimetric chemical tests. Colorimetric tests are when a chemical reagent‚ such as Benedict’s‚ is used on a substance or solution and a certain
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20 MWCO membrane because the urea was too big to pass through. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction. a. Between the attempts to diffuse glucose and albumin through the 200 MWCO membrane‚ the results for both were that glucose was diffuse but the albumin did not. Comparing to my prediction‚ the results were a bit different. I predicted that both were able to diffuse‚ but at the
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j‚ Cosmet. Sci.‚ 54‚ 175-192 (March/April 2003) Effect of mineral oil‚ sunflower oil‚ and coconut oil on prevention of hair damage AARTI S. RELE and R. B. MOHILE‚ Research and Development Department‚ Nature Care Division‚ Marico Industries Ltd.‚ MumbaL India. Accepted for publication April 29‚ 2002‚ Synopsis Previously published results showed that both in vitro and in vivo coconut oil (CNO) treatments prevented combing damage of various
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Cary50 set at 250nm. The pre-dialysis elution pool was pooled together from fractions 17-18 and the flow-through pool was determined to be fractions 2-9. Figure 2: Bradford standard curve created by an assay of seven known dilutions of bovine serum albumin (BSA). The assays were done at 595nm using a
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various food tests and state the justifications. Apparatus : Test-tube ‚ test-tube holder ‚ water bath 95’c ‚ measuring cylinder ‚ white tile ‚ test-tube rack . Materials : Glucose‚ sucrose ‚ fructose ‚ hydrochloric acid ‚ potassium hydroxide ‚ albumin ‚ cooked starch ‚ corn oil ‚ copper (ll) sulphate solution ‚ ascorbic acid solution ‚ DCPIP solution ‚ Millon’s reagent ‚ Sudan lll ‚ tap water‚ ethanol ‚ iodine solution ‚ Benedict’s solution. Observation : Part One : Identification of Carbohydrates
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