bending due to additional mass Corresponding author. Fax: +86 514 7975244. E-mail address: xyhu@yzu.edu.cn (X. Hu). 1 Abbreviations used: AFM‚ atomic force microscope; MC‚ microfabricated cantilever; PZT‚ lead zinconate titanate; BSA‚ bovine serum albumin. 0003-2697/$ - see front matter Ó 2006 Elsevier Inc. All rights reserved.
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2.2 Immunoprecipitation Different protocols were compared so as to choose the one which is compatible with the characteristics of the particular protein of interest [29]. A suitable amount of nuclear extracts was diluted with 100 microliters of chilled PBS‚ 0.1% Triton 100 (to this mixture‚ the following was added to make a final concentration of 1:1000: 0.2 mM PMSF‚ 0.5 mM DTT and 15 (microgram/ microliter). It was made sure that the final concentration of nuclear extract was 0.5-1.0(microgram/
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A Return from BOTOX to Botulism By: T. Claire Frances Date: July 10‚ 2014 Instructor: Linda Maywood‚ MSN‚ CPNP Course Title: HIT 102 Pharmacology of Allied Health Professions BOTOX‚ Dysport‚ Xeomin (onabotulinumtoxinA‚ BTX-A); BTX-B (Myobloc)‚ BTX‚ BoNT‚ BTX A-G‚ C. Botulinum‚ botulinum toxin; and since 2013‚ BTX-H; These are all terms synonymous or derived from the most dangerous toxin known to humankind‚ Botulism. Botulism is the term used most often to describe the serotypes derived
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Activity 1 1. Size and concentration of the solute 2. It was too large to pass‚ as predicted 3. I predicted that the glucose would diffuse since it is small enough but the albumin is much too large to pass through the membrane. The experiment produced this result. Activity 2 1. Neither way requires ATP but facilitated diffusion requires carrier protein molecules for diffusion to occur. 2. By increasing the number of carriers more glucose can pass at a time increasing the rate‚
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conducted to see if the potato cylinders would gain weight or lose weight because of osmosis. The second part was conducted to see if a dialysis bag‚ filled with sodium sulfate and starch solution and placed in an albumin/glucose solution‚ would contain albumin/glucose from the outside or the albumin/glucose solution would contain any sodium sulfate/starch from the inside. The first part of this experiment there is a relationship between the amount of sucrose concentration and final mass. As the sucrose
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MWCO. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membranes. How well did the results compare with your predictions? Glucose was able to diffuse through the 200 MWCO membrane‚ however albumin was not able to diffuse through the 200 MWCO. This is due to the difference of the molecular weight between the two molecules. Glucose has a molecular weight of about 180 g/mol and albumin (a protein composed of 67 amino acids and each amino acid is 67 g/mol)
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Dietary Supplement and Herb Chart Name of Herb Therapeutic Use Scientific Studies That Show Efficacy for Any of the Therapeutic Uses Cautions and Warnings or Side Effects Glucosamine Glucosamine is used in the treatment of- • Osteoarthritis • Knee pain • Back pain • Glaucoma (National Institutes of Health‚ 2011). Lee‚ Woo‚ Choi‚ Ji‚ and Song‚ (2010) through a meta-analysis study examined the effect of glucosamine on osteoarthritis progress. Study findings concluded that
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During a race‚ there is an inherent increase in energy requirements and these requirements are maintained by the many biochemical events happening within the body. This essay explores the metabolic processes that occur at the start of the race‚ 5 minutes into the race and 45 minutes into the race in terms of mobilization‚ biochemical pathways used for degradation of molecules and comparing the yield of ATP of the two types of fuels involved‚ namely carbohydrates and lipids. Carbohydrates‚ which have
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hyponatremia occurs because there is an impairment in renal water excretion‚ due most often to an inability to suppress ADH release. Although the definition may vary among different clinical laboratories‚ hyponatremia is commonly defined as a serum sodium concentration ≤135 meq/L [2]. An overview of the causes of hyponatremia will be presented here (table 1). Most of the individual disorders are discussed in detail separately‚ as are issues related to diagnosis and treatment [1‚3]. (See "Diagnosis
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Hypothesis. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? The glucose diffused through the 200 MCWO membrane while the albumin didnt. 4. Put the following in order from smallest to largest molecular weight: glucose‚ sodium chloride‚ albumin‚ and urea. Sodium chloride urea glucose albumin Activity 2 Simulated Facilitated Diffusion 1. Explain one way in which
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