spectrophotometry • UV-Vis spectroscopy • Fluorimetry Jan 16th‚ 2015 • Fluorescent staining‚ green fluorescent protein • Immmunoassays • Discussion on lab test • Optional tutorial on Ch. 1‚ 2‚ 3‚ 4-1 and 4-2 (8:359:20am) Jan 23rd‚ 2015 - LAB • Error calculations (not on lab test) TEST • 10 minute break • Test on Ch 1‚ 2‚ 3‚ 4-1 and 4-2 (9:30-11:25am) - Lecture and test / Midterm schedule is subject to change at the discretion of Instructor Chapters (N = Notes) Lecture Slides Lecture Slides See lab maunal Ch. 0
Premium Protein Spectroscopy
concentration of the sample‚ the greater the absorbance of light. Beer-Lambert Law states that the amount of light absorbed in colorimetry also depends on the absorbing species‚ the wavelength of light used‚ and the thickness of the sample. For this lab‚ a standard curve is an experimentally measured relationship between concentration and absorbance. Experimental: Materials: 0.1 M Co(NO3)2 (aq) Cuvettes Unknown cobalt(II) solution Volume measuring equipment Device to measure light absorbance
Premium Electromagnetic radiation Absorbance Light
Mass Spectroscopy Mass spectroscopy is a method used in science and industry in order to obtain the masses and relative concentrations of atoms and molecules and to detect isotopes in a sample based on their masses. In order to do this the method requires an instrument called the ‘mass spectrometer’‚ of which‚ is able extract accurate information of the relative masses of isotopes and their relative abundance. This makes the mass spectrometer very useful for applications such as carbon dating
Premium Isotope Chemistry Atom
Year 12 Chemistry 2011 Student Handbook Table of Contents Errors 2 Significant Figures 2 States of Matter in Equations 2 Revision Hints 3 Equations 4 VCAA 2008-2011 Key Skills 6 Unit 3 VCAA Study Design 7 Unit 3 Timetable 8 Unit 3 Work Set Questions 10 Unit 4 VCAA Study Design 11 Unit 4 Timetable 12 Unit 4 Work Set Questions 14 ERRORS When instruments are manufactured‚ there is a specified uncertainty within which the instrument is designed
Premium Chemical reaction Titration Analytical chemistry
Abstract Soil reflectance spectroscopy is a well known technique to assess soil properties rapidly and quantitatively both in point (Spectroscopy) and image (Imaging Spectroscopy (IS)) domains. The quantitative approach has been developed in the past two decades by many researchers and many papers have been published on this subject in the scientific literature. Basically‚ the quantitative approach has been adopted from other disciplines (e.g. food‚ textile‚ pharmaceutical); whereas the mapping
Premium Soil contamination Spectroscopy Pollution
Experiment 2 Quantification of Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A
Premium Analytical chemistry Spectroscopy
Vibration- Rotation Spectroscopy of HCl and DCl Purpose: To determine the fundamental vibration frequency and bond length for H35Cl‚ H37Cl‚ D35Cl‚ and D37Cl and to compare the isotope effects to theoretically predicted values. Introduction Vibration spectroscopy is one of the most important tools for the accurate determination of molecular structure. Vibration spectroscopy also plays an important role in environmental chemistry. For example‚ the albedo of the earth’s atmosphere is controlled by the
Premium Atom Spectroscopy Oxygen
carriedout for the degradation of fabric dyes from aqueous solution using Chara and Chara beads are discussed below.Various analytical techniques like UV-Vis‚ FTIR spectroscopy were used to confirm the decolorization as UV-Vis spectroscopy; FT-IR spectroscopy and toxicity bioassay were truly used [14]. Also‚ Fourier transform infrared spectroscopy (FT-IR) is widely used to confirm the product obtained after decolorization of dye degradation experiments [15]. It helps in determination of reaction
Premium Spectroscopy
List of Chemistry Terms used in daily life α (alpha)‚ β (beta) α- (β-‚ γ-) ray spectrometer α-addition (alpha-addition) α-cleavage (alpha-cleavage) α-decay (alpha-decay) α-effect α-elimination α-expulsion in photochemistry α-oxo carbenes α-particle (alpha-particle) ’A’ value -factorquantity ab initio quantum mechanical methods abatement in atmospheric chemistry abeo- abiological abiotic abiotic transformation absolute activation analysis absolute activity‚ quantity absolute
Premium Chemistry Mass spectrometry Electron
Contents 1. Introduction and literature review 1.1 Polypropylene 1.1.1 Polypropylene crystallization 1.2 Polymer degradation 1.3 Broadband dielectric 1.4 Solubility and the cohesive energy density 1.5 Literature review 2. Experimental method 2.1 Solubility of SN 1010 in various solvents 2.2 Calibration curve 2.3 Preparation of polymer/antioxidant mix for films 2.4 Soxhlet extraction 2.5 Broadband dielectric analysis of films 2.6 Broadband dielectric analysis of molten samples
Premium Polymer Spectroscopy Solubility