Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase‚ which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds‚ causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound’s partition coefficient result in differential retention
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Dove with olive leaf A number of peace symbols have been used in various cultures and contexts‚ one of the most ancient being the olive branch. The symbol of the dove and olive branch was used by early Christians and was later adopted as a secular symbol. It was popularized by Pablo Picasso in 1949 and became widely used in the post-war peace movement. In the 20th century the peace sign was adopted by the Campaign for Nuclear Disarmament. The V hand signal and the peace flag became international
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and that method of knowing is what we call “Candy Crhomatography”. Chromatography by definition according to Webster’s dictionary means “ a method for separating the constituents of a solution (gas or liquid) by exploiting the different bonding properties of different molecules”. In this case‚ the molecules being broken down are the different molecules that make up a certain color. Colors are basically made up of the primary (red‚ blue‚ yellow) and when you mix these colors together is when you get
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It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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Analysis and Separation of Organic Acids in White Wine Using High Performance Liquid Chromatography Atis‚ Arnelson Arwin G. and Gaitos‚ Gerald M. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Performed: December 9‚ 2011 Submitted: December 15‚ 2011 RESULTS AND DISCUSSION solutions containing increasing amounts of the organic acid. These solutions were contained in flasks numbered 5‚ 6‚ 7‚ 8‚ and 9. (Refer to
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CHEM 3152-003 Experiment 2: Fractional Distillation and Gas Chromatography Introduction: The purpose of this experiment was to separate a mixture of ethyl acetate (EtOAc) and toluene by using the method of fractional distillation and to analyze the fractions using gas chromatography (GC). ethyl acetate toluene Distillation is a method known as separating or purifying a liquid or mixture by vaporization and condensation. In a simple distillation‚ the liquids being separated boil below 150 0C
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Lab Design “Investigate the effect of a factor on the number of stomata of a leaf.” Research Question: How do differing leaf heights affect the number/density of stomata of a leaf? Hypothesis Stomata are pores‚ typically found under the leaf (lower epidermis)‚ that control the gas exchange of transpiration‚ where water vapor leaves the plants‚ and carbon dioxide enters. I predict that the stomatal density on high leafs is higher than on low leafs. During photosynthesis the chloroplasts
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Ion Exchange Chromatography Week 10 TAG Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column‚ load your sample and collect the hydronium ion released. 1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the
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Virtual Lab: Plant Transpiration Please complete questions below prior to attempting the lab report for this assignment. Table 1: Total Amount of Water (in mL) Transpired in One Hour Plant Type Normal Conditions (21oC) With Heater (27oC) With Fan (21oC) With Lamp (21oC) Arrowhead 3.6 6.6 7.5 4.0 Coleus 0.9 3.9 6.0 3.0 Devil’s Ivy 2.9 4.1 4.6 3.0 Dieffenbachia 4.1 6.0 7.7 3.9 English Ivy 1.8 3.2 5.1 2.1 Geranium 1.2 5.8 4.7 2.4 Rubber Plant 4.9 6.8 8.4 4.3 Weeping Plant 3.3 4.9 6.1
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Biological Sciences College of Arts and Sciences Visayas State Unversity Visca‚ Babay City‚ Leyte Name: Millama‚ Engene O. Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment
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