Osmolarity TITTLE: practical of estimation of osmolarity in tissues by bathing samples in hypertonic and hypotonic solutions. INTRODUCTION: Osmolarity is the osmolar concentration of plasma and is proportional to the number of particles per liter of solutions shown as (mmol/l). It is derived from the measures Na+ and K+‚ urea and glucose concentrations. Since the volume of solution changes with the amount of solute added also it change in temperature and pressure‚ osmolarity we can say it’s
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Practical Work Nº2: “Different methods to control osmosis” Aim: observe and test the process of osmosis through different kinds of methods: the weigh (potato)‚ the density (beetroot) and under the light microscope (onion). Hypothesis: according to the encyclopedia definition osmosis is the diffusion of a liquid (most often assumed to be water‚ but it can be any liquid solvent) through a partially-permeable membrane from a region of high solvent potential to a region of low solvent potential. Thus
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pressure and same water potential since the two solutions have an equal concentration of water molecules. MATERIALS: 1. Potato slices 2. Elodea leaf/ onion scales 3. Razor blades 4. Digital balance /string /ruler 5. Varying molar of sucrose solutions (0.01 M‚ 0.02 M‚ 0.05 M‚ 0.1 M‚ 0.3 M‚ 0.5 M) 6. Tissue papers 7. Test tubes 8. Watch glass 9. Forceps PROCEDURE: Task 1: The effect of osmosis in potato cells 1. The potato was cut
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LABORATORY 1b - Water Potential II LABORATORY 1b: WATER POTENTIAL II OVERVIEW In this laboratory you will investigate the effect of solute concentration on water potential as it relates to living plant tissues. OBJECTIVES Before you begin this lab you should understand: - the mechanisms of diffusion and osmosis and their importance to cells - the concept of water potential - the relationship between solute concentration‚ pressure potential and the water potential of a solution - the concept
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largest amount of sucrose solution gained more mass than the others. Therefore it had the greatest molarity as more molarity means more percent of change in the mass. The yellow was a hypertonic solution‚ causing it to gain more mass and molarity. 4D- The hypothesis was supported because having no net movement meant that the sucrose solution 0 M would be isotonic in the slice of potatoes. The line from the graph that crosses the x-axis represents the molar concentration of sucrose with water potential
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Guidelines for writing laboratory reports Laboratory reports should be written according to the format below (failure to do so will result in marks being deducted): Formatting Font Type: Times Roman Font size: 12 Spacing: 1.5‚ justified Pages : 5 (minimum) - 10 (maximum) [pages must be numbered] Title page You are required to use the lab report submission page available on the LMS and are to include these details: lab no.‚ title of experiment‚ students’ names and ID‚ date of experiment as well
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solutions on plant cell. Particularly in this experiment we use onion cells. Tissue from an onion is a good first exercise in using the microscope and viewing plant cells. The cells are easily visible under a microscope and the preparation of a thin section is straight forward. An onion is made of layers‚ each separated by a thin skin or membrane. In this exercise you will make a wet mount on a microscope slide and look at the cells of the onion membrane magnified by the high power‚ compound microscope
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energy‚ the molecules go down a concentration gradient. Active transport is the opposite it uses ATPase for energy‚ and molecules move against a concentration gradient. Prediction: When the potato cylinder is placed in a high water potential sucrose solution I think it will increase in mass‚ and length. This is because the solution has a higher water potential than the cells in the cylinder‚ water molecules will travel from a high water potential (The solution) to a low water potential (The potato
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Biological Macromolecules Lab Cells‚ the fundamental units of life‚ are composed of various combinations of organic macromolecules: carbohydrates‚ lipids‚ proteins‚ and nucleic acids. This lab exercise is designed to show you the qualitative tests commonly used to detect their presence. Objectives Test for the presence of monosaccharides by using the Benedict’s test Test for the presence of starch‚ a polysaccharide‚ by using the Lugol’s iodine test Test for the presence of lipids by using the
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percent. For the 0.2‚ 0.4‚ 0.6 0.8‚ and 1.0 Moles of sucrose solutions‚ the bag gained mass. The water had to diffuse across the membrane into the bag to space out the sucrose molecules because the beaker contained no sucrose. The sucrose was unable to diffuse across the dialysis tubing because the molecules were too large for the pores (as referenced in the previous paragraph). This part experiences osmosis because only the water‚ not the sucrose‚ diffused across the membrane to close the gap between
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