Effect of different temperatures on amylase activity. Literature review This study is an attempt to follow the activity of amylase because it has a major role in the life of living organisms and is found abundantly in them. Amylase is a catalytic enzyme which hydrolyzes starch into maltose and dextrin at a certain temperature (Biology.kenyon.edu‚ 2015). In plants such as fruits and vegetables carbohydrates are referred to starch which is polysaccharide and is converted into disaccharide and eventually
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Explain the technique by which copper can be purified. What is a use of pure copper? 19) Explain the processes of phytomining and bioleaching. 20) Why is recycling beneficial to the environment? 21) What % of iron comes from the blast furnace? What effect does this have on its properties? 22) Give some properties of low-carbon‚ high-carbon and stainless steels. 23) Why might an alloy of aluminium be preferable to the pure metal in aeroplane manufacture? 24) What name is given to the metals in
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attempt to observe how enzymes (starch phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate; these factors include temperature‚ pH levels‚ chemical substances
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IA Report 2011 5/31/2011 Enzyme Activity Experiment Introduction 3 AIM 3 Materials 3 Method 4 Result 5 Table 1: 5 Table 2: 5 Graph 1 6 Discussion 6 Conclusion 8 Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9 Introduction Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will go colourless. Here
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Enzymes lower the amount of energy needed in a chemical reaction. This happens because enzymes are catalysts‚ so they speed up the activation rates that occur in living organisms. Without enzymes‚ it would be difficult to break down particles like food in the digestion system. Enzymes are all very specific to what chemical reactions they will work with‚ and the temperature‚ pH‚ and salt concentration have to be a specific levels in order for the enzyme to function. The structure of each enzyme has
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Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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Biology- Change in pH Aim: To investigate the affect that change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known
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Abstract Enzymes are biological catalysts that speed up the rate of chemical reactions by lowering the reactants’ activation energy. The goal of this lab was conducted to determine the optimal temperature for bacterial and fungal Amylases and evaluate how temperature affects the catabolic rate of enzymes. Enzyme reaction rate was measured using an Iodine test in which drops of starch solution with either fungal or bacterial Amylase exposed to different temperatures were mixed with Iodine. Iodine
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1 The Limits of Amylase 2-1-14 Abstract This report explains the purpose of this experiment in a way that conveys information to the reader about Amylase’s ability to withstand acidic or basic pH. To do this‚ two test tubes were both filled with 5mL of a 5% amylase solution. The first one was filled with an acid‚ while the other was filled with a base. After dropping liquid Iodine and Benedict’s solution into each one‚ the tube with a basic pH tested positive for glucose. The acidic solution
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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