Abstract In order to experiment with distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further
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IDENTIFICATION OF UNKNOWN SOLUTIONS USING TLC CHROMATOGRAPHY Purpose: To become familiar with the principles and terminology of TLC chromatography to identify an unknown molecule based on comparisons with known laboratory standards and Rf factors. Background Thin Layer Chromatography: Chromatography is a sophisticated method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that
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Chromatography of Commercial Analgesics Chromatography of Commercial Analgesics CHEMISTRY 200L EXPT 4 PAGE 8 - 11 CHEMISTRY 200L EXPT 4 PAGE 8 - 11 Janna Vernice R. Villalon*‚ Christian V. Villanueva‚ Cyd Vincent L. Zamora Department of Chemistry‚ College of Science *Corresponding author; e-mail: janna.villalon@rocketmail.com Abstract In analyzing the chromatography of analgesics‚ thin layer chromatography (TLC) was used. A very thin (micron) film of silica is coated on a glass
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Experiment 10 Thin Layer Chromatography Purpose! To identify how many components are in a mixture and the identity of a compound in a mixture by comparing the Rf of a known compound with the Rf of an unknown compound. Intodection. In this experiment the thin-layer chromatography the (TLC) will be used to determine the composition of various over-the-counter analgesic. We will be given two commercially prepared TLC plates with a flexible backing and a silica-gel coating with a fluorescent
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the organic phase and the aqueous phase. OH O NaOH + NaI + H2O CH3I Before coming to lab‚ please review the following techniques: "Reluxing a reaction‚" "Extraction and washing‚" "Drying an Organic Solvent‚" "Evaporating an Organic Solvent‚" "Analyzing a Solution by Thin Layer Chromatography (TLC)‚" "Separating Mixtures by Column Chromatography‚" and "Characterizing Compounds by IR." Notice that this lab brings together most of the techniques that we have used this semester. Then complete the
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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Current Regulations for Standardization of Crude Drugs In India a great deal of bulk knowledge exists among ordinary people about the traditional use of herbal medicine. It is difficult to quantify the market size of the traditional Indian system. Since most practitioners formulate and dispense their own recipes. The present annual turnover of product manufactured by large companies is estimated at approximately US $ 300 million compared to a turnover of approximately US $ 2.5 billions for modern
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Introduction: This experiment involves an extraction of a natural product using the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound‚ which is identified by thin layer chromatography (TLC). Eugenol is widely used in dentistry‚ due to its analgesic‚ antiseptic balsamic qualities. It is ideal for curative for pulp hyperemia (the soft‚ sensitive substance underneath the enamel and dentine of a tooth) and pulp its. In dentistry eugenol is also used to
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EXPERIMENT 8 THIN LAYER CHROMATOGRAPHY AND MELTING POINT DETERMINATION: DETECTION OF CAFFEINE IN VARIOUS SAMPLES Additional Resources http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html http://coffeefaq.com/caffaq.html Materials Needed TLC equipment: 1 5-cm x 8-cm TLC plate‚ 2 capillary micropipettes‚ TLC tank and lid‚ ruler‚ UV lamp Extraction Equipment: spatula‚ 2 small test tubes‚ Pasteur pipet and bulb Chemicals: caffeine‚ ethanol‚ dichloromethane‚ TLC solvent (5% acetic acid in ethyl acetate)
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Stereochemistry of Dihydroxylation Reactions Question: Can the stereochemical outcome of dihydroxylation reactions be determined by thin layer chromatography? Overview: In this experiment‚ you will perform two reactions that transform alkenes into diols. While similar in functional group transformation‚ these reactions may lead to stereochemically different products. The two possible products are diastereomers of one another‚ and therefore have different physical chracteristics. After
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