Chemistry Lab Write-Up Title: Paper Chromatography of Powdered Beverage Mix Purpose/Objective: The purpose is to be able to use liquid chromatography to separate dyes in a powdered beverage mix‚ calculate the Rf values for each dye‚ and rank the polarity of each dye present. Hypothesis: If we are to use the liquid chromatography to investigate the different dyes‚ then I think the different dyes will come up having different polarities and Rf values. Procedure/Method: Draw a line across
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melting point*Capillary tube *Tesco aspirin*Melting apparatus * synthesised aspirin | Apparatus for chromatography Materials for chromatography *Chromatography tank *Ethanol dichloromethane *Capillary tubes * Tesco pure aspirin *Weighing boat
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Identification of Plant Pigments by Paper Chromatography Biology 1107L Introduction All living organisms require energy for their chemical processes. The ultimate source of this energy is the sun. Plants convert light energy into the chemical energy of sugars. During photosynthesis pigments are used to capture light energy. Pigments of green plants can easily be separated and identified using a technique called paper chromatography. The purpose of this experiment is to separate
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Ferrocene and Acetylferrocene will be separated using column chromatography. Column chromatography is a separation technique that is used among many disciplines including biology‚ biochemistry‚ microbiology and medicine. Many common antibiotics are purified by column chromatography.1 Column chromatography allows us to separate and collect individual compounds. In this experiment‚ lumen will be the stationary phase‚ and the more polar substance will be retained on the stationary phase longer
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Experiment # 4 Paper Chromatography 1. Why is the chromatogram developed in an essentially closed system? - The chromatogram is developed in a closed system in order to prevent the solvent to evaporate. Most solvents used in the chromatograph are toxic and flammable. It is also put in a close system to reduce the chance of outside factors affect the chromatograph. 2. What is the main advantage of 2-dimensional paper chromatography over a 1-dimensional one? - The
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Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains Azucena‚ Alexis G. An Official Entry to 2010 Regional Science Fair Cluster 2 Physical Science- Individual Negros Occidental National Science High School January 13‚ 2011 Ma. Melanie P. Romero Research IIB Adviser APPROVAL SHEET The research paper attached hereto‚ entitled‚ “Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains”‚ prepared and submitted
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investigation. Clearly explain the separation technique of paper chromatography in terms of partition. Calculate the Rf value of one of the constituent pigments and compare it to the book value. A paper chromatography was used to separate the pigments that make up chlorophyll to analyse‚ identify‚ and quantify the components‚ using paper (stationary phase – the part of the apparatus that does not move with the sample) and chromatography solvent that was 90% petroleum ether and 10% propanol (mobile
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1.5.2.5 Detectors The detectors used in UPLC should be able to handle very fast scanning methods because half-height peak widths of less than one second are usually obtained with columns packed with 1.7 µm particles. The detector must be able to give high sampling rate adequate to capture enough data points across the peak for an accurate and reproducible integration of analyte peak. The dispersion (volume) of the detector flow cell must be minimal to maintain separation efficiency. Conceptually
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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Procedure The procedure stated in Chem 2120 experiment 6 Williamson Ether Synthesis of Phenacetin laboratory manual was followed without any major changes. Data and results Compound Amount used MW (g/mol) Moles Stoichiometry/Comments acetaminophen 0.354 g 151.16 2.34 x 10-3 limiting reagent ethyl iodide 0.3mL 155.97 3.75 x 10-3 1.6 equiv ’s sodium ethoxide 2.6mL 68.05 3.3 x 10-2 catalyst‚ reaction solvent crude product obtained: phenacetin 0.32g
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