by Josh Martin Class Level 1 with Mr Box Abstract: The purpose of this investigation was to determine which out of five readily available chemicals was the most effective at scarifying seeds to improve the rate of germination‚ and what concentration of the most effective chemical was the most efficacious. Ten seeds for each of the five chemicals hydrogen peroxide‚ glucose‚ hydrochloric acid‚ isopropyl alcohol and citric acid were soaked in the solution for approximately twelve hours then subsequently
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme
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bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or cream-colored‚ with irregular borders.} Prepare two slides for gram staining and viewing under a microscope. {Either my gram-stain
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Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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compare to the freezing point of Cyclohexane with the addition of two different concentration of unknown solid. The pure substance of Cyclohexane has defined physical properties. However‚ when a solute is added these properties are determined by the amount of solute added‚ also known as colligative properties. The temperature of the freezing point was lowered when the solid was added and it decreased as the concentration of the solid increased. The molality of the solute was determined‚ which was then
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Thermodynamics- Enthalpy of Reaction and Hess’s Law December 5‚ 2011 Kylie Case‚ Emma McKee‚ Rebecca Smith Purpose: In this lab‚ the purpose was to verify Hess’s Law. Theory: Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The first being enthalpy of reaction‚ ΔHrxn‚ which is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant
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Research & Design in Psychology / G Faculty of Health‚ University of Canberra LAB REPORT COVER SHEET Instructions: 1. Complete these details and the declarations electronically. 2. Insert this sheet at the start of your lab report. 3. Submit the entire assignment‚ including this coversheet‚ as one file via the lab report drop-box on Moodle. 4. For more information‚ see Lab report guidelines. |STUDENT NAME:
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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The Folin assay overestimated the chymotrypsin concentration because of the difference in the properties of BSA and chymotrypsin. The different amino acid composition could have had an effect on how much the Folin reagent was reduced which causes the observed colour change. Too many of these amino acids which have this reducing ability in chymotrypsin could have led to the higher observed concentration seen in this
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(2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab specifically does not require the knowledge of what each organism does when introduced to another living organism‚ like a human. Figuring out the type of bacteria could help with further experiments. The idea of the unknown bacteria lab is to show
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