Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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October 29‚ 2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added
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fire-related deaths? What is the major cause of residential fires? a) 2‚640 people died in a fire related death‚ not including firefighters. Smoking is the leading cause of fire-related deaths. Cooking is the Primary cause of residential fires. 2. How many people are bitten by dogs each year? How many people die from dog bites each year? What is the most common age for people to get dog bites? a. 4.5 million People are bitten by dogs every year. 10 to 20 people die each year from dog
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where an actual attack occurs and the IDPS isn’t alarmed at all. The least desirable would definitely be the false negative‚ if an attack does occur and the IDPS doesn’t even alarm well what’s the point in even having it if it isn’t going to alarm. 2. What is a honeypot? How is it different from a honeynet? A honeypot is a decoy system that will lure potential hackers away from the networks critical systems. A honeynet is where the system subnet is constantly changing and it lures attackers away
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distributed denialofservice‚ and mail bomb. b. A virus must be executed such as opening an infected email attachment while a worm can be initiated with or without the user downloading or executing the file. c. A Trojan horse carries neither a virus or worm. 2. Why does polymorphism cause greater concern than traditional malware? How does it affect detection? A polymorphic virus is a complicated computer virus that affects data types and functions. It is a selfencrypted virus designed to avoid detection by a scanner
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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KOLEJ MATRIKULASI MELAKA 78300 MASJID TANAH‚ MELAKA Guidelines for Biology Experiments 1.0 Attendance 1.1 Attendance to practical sessions is COMPULSORY. 1.2 If you cannot come to the class due to ill health or emergency excuses‚ please inform the lecturer EARLIER‚ so that another practical session can be arranged for you ON THE SAME WEEK. 2.0 Lab coat 2.1 Wearing lab coat is COMPULSORY. 2.2 Put on the lab coat throughout the class. 3.0 Jotter 3.1 Jotters should contain the summary on the PROCEDURES
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