0.032 L-Proline was stirred in 8 ml of acetone for 15 minutes then 0.12 g of 4-Nitrobenzaldehyde was added and the mixture was stirred for 1 hour and half. Then the mixture was treated with 8 ml of saturated of aqueous ammonium chloride solution and extraction with ethyl acetate was made, and the organic layer was separated and dried with (MgSO4 ) then by filtered.
To purified the product Thin-layer chromatography (TLC), 15 test tube were used to collect the