Accumulation of Vasicine and Vasicinone in Tissue Cultures of Adhatoda Vasica and Evaluation of the Free Radical-Scavenging Activities of the Various Crude Extracts
Food Chemistry xxx (2010) xxx–xxx
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Food Chemistry journal homepage: www.elsevier.com/locate/foodchem
Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts
G. Roja a,⇑, B.H. Vikrant b, Santosh Kumar Sandur c, Asmita Sharma d, K.K. Pushpa d a Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India Department of Biotechnology, Chauhan Institute of Science, Mithibai College, University of Mumbai, Mumbai 400 056, India c Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India d Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India b a r t i c l e
i n f o
a b s t r a c t
The indigenous medicinal plant, Adhatoda vasica (L.) Nees. (Acanthaceae), commonly known as vasaka, is used as a herbal remedy for allergen-induced bronchial obstruction, asthma, and tuberculosis and possesses hepatoprotective activity. This plant is a natural source of vitamin C. Tissue cultures of A. vasica, initiated on Murashige and Skoog’s medium supplemented with various plant growth regulators, showed the presence of alkaloids, vasicine and vasicinone. Water extracts of shoot cultures contained high levels of these alkaloids. The vasicine and vasicinone contents in these extracts were 5.98% and 5.2% of dry weight and the water extracts of the selected elite parent plant contained 3–4% dry weight of vasicine. The methanolic extracts of the parent plant and shoot cultures showed quantitative differences in the level of both vasicine and vasicinone. Maximum free radical-scavenging activity of DPPH radicals was observed in the water extracts. Ó 2010 Elsevier Ltd. All rights reserved.
Article history: Received 23 April 2010 Received in revised form 26 October 2010 Accepted
Contents lists available at ScienceDirect
Food Chemistry journal homepage: www.elsevier.com/locate/foodchem
Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts
G. Roja a,⇑, B.H. Vikrant b, Santosh Kumar Sandur c, Asmita Sharma d, K.K. Pushpa d a Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India Department of Biotechnology, Chauhan Institute of Science, Mithibai College, University of Mumbai, Mumbai 400 056, India c Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India d Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India b a r t i c l e
i n f o
a b s t r a c t
The indigenous medicinal plant, Adhatoda vasica (L.) Nees. (Acanthaceae), commonly known as vasaka, is used as a herbal remedy for allergen-induced bronchial obstruction, asthma, and tuberculosis and possesses hepatoprotective activity. This plant is a natural source of vitamin C. Tissue cultures of A. vasica, initiated on Murashige and Skoog’s medium supplemented with various plant growth regulators, showed the presence of alkaloids, vasicine and vasicinone. Water extracts of shoot cultures contained high levels of these alkaloids. The vasicine and vasicinone contents in these extracts were 5.98% and 5.2% of dry weight and the water extracts of the selected elite parent plant contained 3–4% dry weight of vasicine. The methanolic extracts of the parent plant and shoot cultures showed quantitative differences in the level of both vasicine and vasicinone. Maximum free radical-scavenging activity of DPPH radicals was observed in the water extracts. Ó 2010 Elsevier Ltd. All rights reserved.
Article history: Received 23 April 2010 Received in revised form 26 October 2010 Accepted
References: Amin, A. H., & Mehta, D. R. (1959). A bronchodilator alkaloid (vasicinone) from Adhatoda vasica Nees.. Nature, 184, 1317. Atal, C. K. (1980). Chemistry and pharmacology of vasicine: A new oxytoxic and abortifacient pp. 93–103. Jammu Tawi, India: Regional Research Laboratory. Barry, V. C., Conalty, M. L., Rylance, H. J., & Smith, F. R. (1955). Antitubercular effect of an extract of Adhatoda vasica. Nature, 176, 119–120. Please cite this article in press as: Roja, G., et al. Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts. Food Chemistry (2010), doi:10.1016/j.foodchem.2010.11.115