Bacteria Morphology
MBK – Lab Report
Name: Katie Nave
Bacterial Morphology
Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms :
Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. It was hard to tell what I was looking at. There were a number of amoeba shaped cells of varying sizes. There were five darker areas circular in nature. There were also three very large, odd shaped areas with distinct edges. Part 4: Direct Staining: Record your observations for each sample. Slide One: There were two distinct clusters that were easily noted. All the cells were cocci. Some of the cells were huge while others were practically nonexistent. Slide Two: There were layers of cells. The cells seemed to all be rectangular in nature with varying sizes. There was a distinct circular nucleus in each cell. Slide Three: It seem like there were a lot of layers of “stuff.” There was a matrix of sorts consisting of all kinds of shapes and sizes. I wasn’t able to find one specifc layer to look at. I think I was looking at chains of cocci or bracillus. Part 5: Indirect Staining: Examine the stained specimens and record your results. These were much easier to see, especially the plaque stain. I saw distinct chains of varying lengths made up of both cocci and bracillus cells. The messy matrix I noted earlier was no longer an issue. The cheek and yeast smear were also much cleaner. I saw the same shapes as before, just with sharper outlines.
Questions:
A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages of using 70% alcohol? The disadvantages? 70% alcohol mixtures are capable of killing most bacteria within five minutes. However, the mixture is ineffective against spores and has limited effect on viruses. Additionally, the 70%
Name: Katie Nave
Bacterial Morphology
Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms :
Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. It was hard to tell what I was looking at. There were a number of amoeba shaped cells of varying sizes. There were five darker areas circular in nature. There were also three very large, odd shaped areas with distinct edges. Part 4: Direct Staining: Record your observations for each sample. Slide One: There were two distinct clusters that were easily noted. All the cells were cocci. Some of the cells were huge while others were practically nonexistent. Slide Two: There were layers of cells. The cells seemed to all be rectangular in nature with varying sizes. There was a distinct circular nucleus in each cell. Slide Three: It seem like there were a lot of layers of “stuff.” There was a matrix of sorts consisting of all kinds of shapes and sizes. I wasn’t able to find one specifc layer to look at. I think I was looking at chains of cocci or bracillus. Part 5: Indirect Staining: Examine the stained specimens and record your results. These were much easier to see, especially the plaque stain. I saw distinct chains of varying lengths made up of both cocci and bracillus cells. The messy matrix I noted earlier was no longer an issue. The cheek and yeast smear were also much cleaner. I saw the same shapes as before, just with sharper outlines.
Questions:
A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages of using 70% alcohol? The disadvantages? 70% alcohol mixtures are capable of killing most bacteria within five minutes. However, the mixture is ineffective against spores and has limited effect on viruses. Additionally, the 70%