Catalase And Temperature Lab Report
The Effect of catalase reaction on temperature
Table of Contents:
Bibliography ………………………………………………………………………………………………….. Page 1
Introduction……………………………………………………………………………………………………Page 2-3
Methods/ Procedures……………………………………………………………………………………..Page 4-5
Results ………………………………………………………………………………………………………….. Page 6
Analysis of Data ……………………………………………………………………………………………. Page 6
Graph …………………………………………………………………………………………………………... Page 7
Conclusion…………………………………………………………………………………………………… Page 8
Bibliography:
Cooper, Geoffrey M. “Enzyme Lab.” Enzyme Lab. ASM Press and Sinauer Associates, Inc. n.d. Web. 13 Nov. 2013.
“Catalase.” Catalase. Princeton Edu, n.d. Web. 13 Nov. 2013.
Chelikikani, I. …show more content…
One molecule of catalase can convert 40 million molecules of hydrogen peroxide to water and oxygen. Hydrogen peroxide is a harmful product of normal processes to prevent damage to cells and tissue so it must be converted into less hazardous substances. Hydrogen peroxide is said to denature cells meaning to kill cells. Temperature can affect the rate of enzyme reaction, according to the laws of thermodynamics. As the temperature increases the rate the reaction increases. High temperatures can denature the enzyme. Denature means to kill or destroy the enzyme. This will result in the enzyme to lose its function. The role of catalase enzyme in the cell is to break down hydrogen peroxide in the metabolism. This is in a process of two steps. The first step is one molecule of hydrogen peroxide oxidizes the tissue. The second step is when the hydrogen peroxide molecule is used as a reluctant to restart the enzyme causing it to create water and …show more content…
I measured the fizz in the graduated cylinder. I did 5 temperatures and 5 trials for each temperature and calculated an average. After calculating the average for each temperature I compared the averages and learned that each temperature had a different reaction rate. For safety precautions I wore gloves, goggles, and carefully handled the hydrogen peroxide.
Procedures:
Place 1 ml of the 3% hydrogen peroxide solution into a clean 10ml graduated cylinder.
Using forceps and sharp knife/scalpel cut 0.1g of chicken liver (this is approximately half the size of a 10c coin) and add it to the measuring cylinder
Label measuring cylinder 1 – (temperature of chicken liver)
Push the piece of liver into the hydrogen peroxide with a stirring rod. Observe what happens.
Leave the reaction for 4 minutes and then measure how high the bubbles rose.
Record the data
Repeat steps 1-6 for the remaining temperatures and trials
Calculate Averages for each temperature
Results:
Analysis of
Table of Contents:
Bibliography ………………………………………………………………………………………………….. Page 1
Introduction……………………………………………………………………………………………………Page 2-3
Methods/ Procedures……………………………………………………………………………………..Page 4-5
Results ………………………………………………………………………………………………………….. Page 6
Analysis of Data ……………………………………………………………………………………………. Page 6
Graph …………………………………………………………………………………………………………... Page 7
Conclusion…………………………………………………………………………………………………… Page 8
Bibliography:
Cooper, Geoffrey M. “Enzyme Lab.” Enzyme Lab. ASM Press and Sinauer Associates, Inc. n.d. Web. 13 Nov. 2013.
“Catalase.” Catalase. Princeton Edu, n.d. Web. 13 Nov. 2013.
Chelikikani, I. …show more content…
One molecule of catalase can convert 40 million molecules of hydrogen peroxide to water and oxygen. Hydrogen peroxide is a harmful product of normal processes to prevent damage to cells and tissue so it must be converted into less hazardous substances. Hydrogen peroxide is said to denature cells meaning to kill cells. Temperature can affect the rate of enzyme reaction, according to the laws of thermodynamics. As the temperature increases the rate the reaction increases. High temperatures can denature the enzyme. Denature means to kill or destroy the enzyme. This will result in the enzyme to lose its function. The role of catalase enzyme in the cell is to break down hydrogen peroxide in the metabolism. This is in a process of two steps. The first step is one molecule of hydrogen peroxide oxidizes the tissue. The second step is when the hydrogen peroxide molecule is used as a reluctant to restart the enzyme causing it to create water and …show more content…
I measured the fizz in the graduated cylinder. I did 5 temperatures and 5 trials for each temperature and calculated an average. After calculating the average for each temperature I compared the averages and learned that each temperature had a different reaction rate. For safety precautions I wore gloves, goggles, and carefully handled the hydrogen peroxide.
Procedures:
Place 1 ml of the 3% hydrogen peroxide solution into a clean 10ml graduated cylinder.
Using forceps and sharp knife/scalpel cut 0.1g of chicken liver (this is approximately half the size of a 10c coin) and add it to the measuring cylinder
Label measuring cylinder 1 – (temperature of chicken liver)
Push the piece of liver into the hydrogen peroxide with a stirring rod. Observe what happens.
Leave the reaction for 4 minutes and then measure how high the bubbles rose.
Record the data
Repeat steps 1-6 for the remaining temperatures and trials
Calculate Averages for each temperature
Results:
Analysis of