Differential Staining
Differential Staining
Purpose: The purpose of this experiment was to become familiar with subtypes of culture media and the uses for each, learn and employ the streak and pour dish techniques, and generate a pure culture of a specific organism.
Set Up: For this experiment I needed: 1 Distilled water, 1 Paper towels, 1 10%-bleach or 70% alcohol solution, 1 Zip bag, 1 Pan to heat agar, 1 Isopropyl alcohol (rubbing alcohol), 1 Cultures: S. epidermidis and L. acidophilus, 1 Gloves, Disposable, 1 Pencil, marking, 11 Petri dish, 60 mm, 2 Candles (flame source), 1 Thermometer-in-cardboard-tube,6 Test Tube(6), 16 x 125 mm in Bubble Bag, 1 Test tube holder, 1 Test-tube-rack-6x21-mm, 1 Pipet Graduated Small (5 mL), 1 Baker’s Yeast Packet – Saccharomyces cerevisiae, 1 Agar, MRS - 18 mL in Glass Tube, 4 Agar, Nutrient - 18 mL in Glass Tube, 1 Broth, Nutrient - 5 mL in Glass Tube, 2 Inoculation Loop, Plastic, 1 Mask with Earloops (11) in Bag 5" x 8"
of Individual Colonies
Methodology: Exercise 1: Isolation Using the Pour Plate Method Part I: Preparation of Solid Media
1. Disinfect the work area.
2. Melt the agar tubes. Refer to the Preparation of Solid Media section in the Introduction for further instruction.
3. Leave the 18 mL tube of MRS agar in hot water (50°C) for use in Part II.
4. Use the marking pencil to label the bottom of one Petri dish S. epidermidis. Pour one half (9 mL) of the contents of a tube of nutrient agar into the S. epidermidis Petri dish and the other half into the bottom of an unmarked Petri dish. Cover the dishes and allow them to solidify for use in Part IV.
5. Pour the remaining melted nutrient agar into the unmarked Petri dishes (half a tube per dish). Cover the dishes and allow them to solidify for use in Part III. Part II: Isolation Using the Pour Plate Method
1. Disinfect the work area.
2. Label the bottom surface of three sterile Petri dishes L. acidophilus #1, #2, and #3, respectively.
3. Disinfect three test tubes
Purpose: The purpose of this experiment was to become familiar with subtypes of culture media and the uses for each, learn and employ the streak and pour dish techniques, and generate a pure culture of a specific organism.
Set Up: For this experiment I needed: 1 Distilled water, 1 Paper towels, 1 10%-bleach or 70% alcohol solution, 1 Zip bag, 1 Pan to heat agar, 1 Isopropyl alcohol (rubbing alcohol), 1 Cultures: S. epidermidis and L. acidophilus, 1 Gloves, Disposable, 1 Pencil, marking, 11 Petri dish, 60 mm, 2 Candles (flame source), 1 Thermometer-in-cardboard-tube,6 Test Tube(6), 16 x 125 mm in Bubble Bag, 1 Test tube holder, 1 Test-tube-rack-6x21-mm, 1 Pipet Graduated Small (5 mL), 1 Baker’s Yeast Packet – Saccharomyces cerevisiae, 1 Agar, MRS - 18 mL in Glass Tube, 4 Agar, Nutrient - 18 mL in Glass Tube, 1 Broth, Nutrient - 5 mL in Glass Tube, 2 Inoculation Loop, Plastic, 1 Mask with Earloops (11) in Bag 5" x 8"
of Individual Colonies
Methodology: Exercise 1: Isolation Using the Pour Plate Method Part I: Preparation of Solid Media
1. Disinfect the work area.
2. Melt the agar tubes. Refer to the Preparation of Solid Media section in the Introduction for further instruction.
3. Leave the 18 mL tube of MRS agar in hot water (50°C) for use in Part II.
4. Use the marking pencil to label the bottom of one Petri dish S. epidermidis. Pour one half (9 mL) of the contents of a tube of nutrient agar into the S. epidermidis Petri dish and the other half into the bottom of an unmarked Petri dish. Cover the dishes and allow them to solidify for use in Part IV.
5. Pour the remaining melted nutrient agar into the unmarked Petri dishes (half a tube per dish). Cover the dishes and allow them to solidify for use in Part III. Part II: Isolation Using the Pour Plate Method
1. Disinfect the work area.
2. Label the bottom surface of three sterile Petri dishes L. acidophilus #1, #2, and #3, respectively.
3. Disinfect three test tubes