Enzyme Lab Report
In this enzyme lab, (insert creative name), the enzyme catalase was observed under varying conditions in order to interpret what prohibits and inhibits the functioning of certain enzymes in chemical reactions. Enzymes aid in chemical reactions by speeding up the time it takes for the reaction to occur, without getting “used up” throughout the process. Catalase, the specific enzyme used in this lab, is a protein abundant in the liver and red blood cells. It enhances the breakdown of hydrogen peroxide, the substrate, into oxygen, the element measured in the lab, and water (Wiles, 2016). This lab included three different procedures, all preformed with an assay system, in order to gather information on the kind of environment ideal for catalase.
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It was predicted that over time the oxygen would increasingly build up. The second procedure focused on the effects of pH levels on the enzyme activity. For this section, catalase was measured into a test tube along with the specific pH being tested; pH 4, 6, 7, 8, and 10 were used. After preparing the enzyme and pH buffer, hydrogen peroxide solution was added allowing the reaction to occur over a minute time span. It was predicted that the pH of 7 would be considered the optimum pH and anything more acidic or basic would denature the enzyme slowing down the reaction. Lastly, in the third part of the lab, temperature’s effect on an enzyme’s activity was observed. In this procedure, the enzyme was mixed in one test tube with a neutral pH while the hydrogen peroxide was poured into a separate test tube. The test tubes were then placed in different environments; 0 °C, 25 °C, 37 °C, 50 °C, and 70 °C. When the test tubes were at their required temperatures, the hydrogen peroxide was poured into the other test tube and the reaction was recording for one minute. It was predicted that at 37 °C the reaction would occur fastest therefore more oxygen would have built up in this
It was predicted that over time the oxygen would increasingly build up. The second procedure focused on the effects of pH levels on the enzyme activity. For this section, catalase was measured into a test tube along with the specific pH being tested; pH 4, 6, 7, 8, and 10 were used. After preparing the enzyme and pH buffer, hydrogen peroxide solution was added allowing the reaction to occur over a minute time span. It was predicted that the pH of 7 would be considered the optimum pH and anything more acidic or basic would denature the enzyme slowing down the reaction. Lastly, in the third part of the lab, temperature’s effect on an enzyme’s activity was observed. In this procedure, the enzyme was mixed in one test tube with a neutral pH while the hydrogen peroxide was poured into a separate test tube. The test tubes were then placed in different environments; 0 °C, 25 °C, 37 °C, 50 °C, and 70 °C. When the test tubes were at their required temperatures, the hydrogen peroxide was poured into the other test tube and the reaction was recording for one minute. It was predicted that at 37 °C the reaction would occur fastest therefore more oxygen would have built up in this