Enzyme Reaction Lab Report

The environmental factors that effected the rate of enzyme reactions were the enzyme concentration, pH, and temperature. These environmental factors help enzymes break down the poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in the hydrogen peroxide. We also tested another enzyme reaction for pH. In this test we learned that high pH equal high reaction rate. The 4 items tested were acetic acid (vinegar), sodium bicarbonate, water, and NaOH. The two with the lowest pH levels
were water and acetic acid. Both of these had reaction rates of 2 and 3. The two highest pH levels were sodium bicarbonate and NaOH. Both of them had reaction rates of 4 and 5. This concludes that high pH equals high reaction rate. The last test for an enzyme reaction was temperature. In this test we learned that heat helps speed up a reaction to a point. We tested a reaction for a cold liver with hydrogen peroxide, and the reaction rate for it was 1. After that we tested a hot liver with hydrogen peroxide and the reaction rate was 5. This concludes that when the substance is in a cooler condition the reaction rate is slower. But, when the substance is in a warmer condition the reaction rate is fast. The enzyme concentration, pH, and temperatures, all play a role that effects a way an enzyme reaction is acted.