Preview

Escherichia Coli and Staphylococcus Albus

Good Essays
Open Document
Open Document
1035 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Escherichia Coli and Staphylococcus Albus
Escherichia coli and Staphylococcus albus

Some bacteria's require a certain amount of light in order to function properly and there fore survive. Escherichia coli is found in the gut and intestines of mammals.
Staphylococcus albus is generally a skin dwelling bacteria

Aim: To test whether the amount and type of light that the bacteria's E.coli and S.albus are subject to causes changes in their growth rates and habits.

To test whether the growth rates and habits of the bacteria's E. coli and S. albus are subject to change when to a varying amount and type of light.

Hypothesis: that the different types of light will affect the growth rate of the bacteria's. From prior knowledge and assumption of the environment, it is believed that higher temperatures will assist in the growth rate in the agar plates. Therefore it is believed that the agar plates placed in full light will produce more bacteria.
Due to the type of light used for the full light part of the experiment there will be higher temperatures and therefore grow better than the no light and day light plates which are at lower temperatures.

Equipment and Materials:
1 6 agar plates
2 E. coli and S. albus bacteria
3 Light
4 Day light
5 Cupboard
6 Dropper
7 Labels
8 Adhesive tape

Method:
1. using three agar plates make a lawn culture with E.coli, do the same with the other three agar plates with S.albus.
2. Separate into the following groups - Daylight i. E.coli (1) ii. S.albus (2) - No light i. E.coli (3) ii. S.albus (4) - Full light i. E.coli (5) ii. S.albus (6)
3. Place all plates in cupboard and incubate for 3 days undisturbed.

4. Place each pair of plates in the appropriate location - Full light in an area that allows it to be in constant effect of the light and undisturbed with the exception of the data recording - No light in an area which allows it to be in almost constant complete darkness and undisturbed with exception of data recording - Day

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Lab 4

    • 890 Words
    • 4 Pages

    Name and Course Section: Avital Gershtein, Section 701 Title: Aseptic Technique & culturing Microbes - Lab # 4…

    • 890 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    Describe the precise chemical (or physical) reactions that produce these patterns of bacterial growth in each case. (That is, say WHY these results occur). Some of the information is available in your lab manual but for others you may have to do some research to find out the answers.…

    • 648 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    BIO20002 Prac Report 2 1

    • 915 Words
    • 8 Pages

    This experiment is aimed to examine the effects of environment such as Oxygen, Temperature, pH and Osmotic Limitations on the growth of a various kind of bacteria.…

    • 915 Words
    • 8 Pages
    Powerful Essays
  • Good Essays

    The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the effect of light intensity on the rate of photosynthesis in a plant called Elodea. Elodea is an easy plant to use for this experiment because it is cheap, abundant, and easy to take care of. This photosynthetic organism needs to be kept in an aerated freshwater tank. To do this experiment, put the Elodea in a beaker of freshwater. Anchor the Elodea using a small weight like a paperclip so that the plant remains in the same position and up right through the entire experiment. Place the beaker of water containing the Elodea in front of a light bulb. In between the beaker and the light bulb, there should be a separate beaker of water that is used to absorb any heat produced by the light. This way the experiment will not be affected by another environmental factor, temperature. Every thirty seconds for five minutes, count the number of bubbles given off by the Elodea. After the first five minutes, move the beaker containing the Elodea about ten centimeters back and let it stand for two minutes so that it can adjust to the new intensity of the light. Count the bubbles released every thirty seconds for five minutes. Move the Elodea back ten more centimeters, let it adjust for two more minutes, then count the number of bubbles…

    • 633 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Bacteria grown in a closed system show a specific growth pattern called the growth curve which consists of four phases. The lag phase, which is a period of slow growth; exponential phase, period of maximum growth; stationary phase, where nutrients become the limiting factor making the growth rate equal to the death rate and the death phase where organisms die faster than they are replaced. It is important to know how fast a microbe grows in order to know bactericidal or antibiotic concentrations, temperature and ph at which they stop growing. Evaluating a growth curve gives you a perspective of the generation time and the mean growth rate constant which would help you estimate the minimum, maximum and optimum growth temperature of the microbe. In this experiment you would be able to see the duration of each phase, the mean growth rate constant (k) is used to measure how fast cells are dividing in a culture, generation time, optical density (OD) which s the measure of the amount of light absorbed by a suspension of bacterial cells, and the organism’s minimum, maximum, and optimum temperatures…

    • 1189 Words
    • 5 Pages
    Satisfactory Essays
  • Powerful Essays

    Introduction: The type environment that bacteria live in is essential to their growth. In order to establish what environment for V. natriegens is optimal, the generation time of the bacteria under several different environments, must be obtained. The type of system used for this experiment was a closed-growth system. A closed-growth system is a type of environment where there is no interaction with the outside environment. Nutrients are not added to the environment and the waste products are not removed. The advantage of growing bacteria in a closed system is that the four different phases of growth are able to be observed. The four phases are growth include the following: Lag Phase- bacteria adjust to environment, no growth observed; Log Phase- bacteria have adjusted to environment and being growing; Stationary Phase- nutrients in the environment have begun to run out and waste has begun to accrue. The environment is being to be unfavorable and the growth rate of the bacteria begins to equal the death rate of the bacteria; Death Phase- All the nutrients have been consumed and the waste is turning toxic, the bacteria begin to die. By observing these phases, scientists are able to construct a graph that illustrates these phases, called a bacterial growth curve. Scientists are…

    • 1366 Words
    • 6 Pages
    Powerful Essays
  • Powerful Essays

    Bacteria grows by binary fission. One bacteria becomes two, two bacteria becomes four bacteria and it so goes on. A lot of changes occur in the bacterial cell during growth and due to this reason it is difficult to measure growth quantitatively. Growth is therefore usually measure by changing one or two easily measure parameters which will ignore the complicated cellular change. Therefore different type of analysis may yield different results and the measure of cell growth is only a crude method.…

    • 1424 Words
    • 7 Pages
    Powerful Essays
  • Powerful Essays

    During this exercise the population growth of the bacteria Escherichia coli (E.coli) will be measured. If the conditions are right, the E.coli population will grow more rapidly the longer it stays in that area. Turbidity, meaning cloudiness, will be how the tubes are measured to give the results of bacterial growth. The more turbid the broth the more bacteria count the tube will contain. For this particular exercise, a broth has been…

    • 970 Words
    • 4 Pages
    Powerful Essays
  • Satisfactory Essays

    R Fascians Experiment

    • 278 Words
    • 2 Pages

    In the first experiment, the bacteria R. fascians and R. corynebacterioides grew equally well in all conditions of light, except ultraviolet light. We tested this by comparing the petri dishes to the petri dishes that grew without light. Here, the exposure time was constant for all the lights and no light, which is 48 hours. The light sources were different. However, none of the lights had an effect on the bacteria, for the bacteria just continued to grow normally. The only exception was that UV light killed both bacteria completely. In the second experiment, we decided to take the bacteria and expose it to UV light for shorter durations, starting from 5 minutes, 10 minutes, and so on until 24 hours. For 5 minutes, R. fascians had an average…

    • 278 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Bacterial Growth Lab

    • 1203 Words
    • 5 Pages

    Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution, countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell density) vs. time.…

    • 1203 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Bacteria Growth Lab

    • 886 Words
    • 4 Pages

    The medium for this experiment has been prepared and aliquoted into glass flasks which were sealed with bung foam and tin foil and autoclaved overnight. A culture of Vibrio natriegens in the lag phase were also provided by the university. Each of the students were provided with a 10ml culture of Vibrio natriegens, 50ml control flask of marine broth, 50ml experimental flask and a spectrophotometer. Water baths with temperatures of 30¬oC, 37oC and 40oC were also provided to reach optimum experimental…

    • 886 Words
    • 4 Pages
    Good Essays
  • Good Essays

    The Hypothesis created states, “If the elodea plant is closer to the light then, there will be a higher amount oxygen and glucose produced because the light intensity is stronger closer to the lamp.”. The hypothesis was refuted since the majority of oxygen bubbles at 76 cm away as opposed to 10cm. Plants need oxygen, water, and carbon dioxide to survive and produce Photosynthesis, which is why the hypothesis thought the more light equals more bubbles. The hypothesis was refuted seeing as at the closest point to the light there was less bubbles than further away.…

    • 618 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    The plant requires light, carbon dioxide and water to partake in photosynthesis. Photosynthesis is crucial to the survival of the plant and all organisms that rely on the plant for food as it enables the plant to produce glucose which it uses to produce energy (ATP) to enable the plant to grow and survive. If one of these requirements were to be increased in quantity, then logically, the rate of photosynthesis in the plant would be increased as well due to the increased amount of resources. In this experiment, the requirement that was increased was light measured in the form of intensity. It is expected that light intensity will affect the plant’s rate of photosynthesis by increasing it due to the increased amount of resources that the plant now has. The rate of photosynthesis increases as light intensity is increased further; however, the rate of photosynthesis is eventually limited by some other factor. (Royal Society of Chemistry, no date)…

    • 1608 Words
    • 7 Pages
    Powerful Essays
  • Better Essays

    4. Life is is a characteristic that distinguishes objects that have signaling and self-sustaining processes from those that do not,…

    • 810 Words
    • 4 Pages
    Better Essays
  • Good Essays

    For the purpose of this exercise I will focus on E.coli. Pathogenic Escherichia coli will be discussed since it is a common, but dangerous bacterium. E.coli in humans is found in the intestines. This bacterium is very durable, meaning that it is well-adapted to its habitat. For example, it can grow with glucose being the only food source. This bacterium can also grow with or without O2. If located in anaerobic habitat it can it will use the fermentation process producing mixed acids and gases (Todar 2012). This bacterium has shown that it can also use anaerobic respiration when NO3 or NO2 is available. Chemicals, pH, temperature, are a few signals that determines how E.coli will respond (Todar 2012). When it senses a change in the environment it can swim toward or away from anything useful or harmful. Temperature can also affect E.coli. A change in temperature allows E.coli to change pore diameter of its outer membrane to accommodate certain nutrients, or to exclude something harmful. E.coli also rations its nutrient supply by taking in account how much is available in its environment. This means that it will not take in nutrients unless…

    • 402 Words
    • 2 Pages
    Good Essays