Fig. 7: A Case Study

Fig.7. (A) statistical results of modified Garcia scores after 1day to 7day in mice.The results showed that the Necrostatin-1 group was higher than that of the Dmso group and the ICH group,on the 2 days after operation, the score of Necrostatin-1 group was increased (*P < 0.05 vs Dmso group),4day, 7day,Necrostatin-1 group is higher than ICH group ( *P < 0.05 vs ICH group).(B)Statistical results from 1 days to 7 days after test Corner in mice,In 2 days, 4-7 days,Necrostatin-1 group of mice, the number of times turn to the injured side reduced (*P < 0.05 vs ICH group).(C)Statistical results from 1 days to 7 days after test Rota-rod in mice.The results showed that the time of staying in the Necrostatin-1 group was longer in 2-7 days (*P < 0.05
Brain edema is an increase in brain tissue water content, resulting in increased volume of the brain and intracranial pressure [23].In patients with intracerebral hemorrhage, edema is formed within a few hours after intracerebral hemorrhage,1 to 2 weeks reach the peak[24].A Study[25] showed that, in the large animal model of cerebral hemorrhage (such as pigs), edema around the hematoma is mainly distributed in the white matter. The formation of brain edema after intracerebral hemorrhage, which may lead to increased intracranial pressure, cerebral hernia. In the present study, the water content of the brain tissue around the hematoma after intracerebral hemorrhage was significantly increased, the water content of Necrostatin-1 group was decreased, and there was a significant difference between the Necrostatin-1 group and the cerebral hemorrhage group, indicate that Necrostatin-1 can reduce brain edema after the cerebral
Membrane integrity and the permeability of the plasma membrane are important features of cell necrosis, Propidium permeability can be used as a marker of cell necrosis. Propidium iodide is a molecular weight of 668 nucleic acid staining material when combined with RNA or DNA emit bright red fluorescence. We used PI markers to find the second day of the red cell lysis solution, the processes of glial cells were significantly reduced, the cell appeared atrophy, and the permeability of the cell membrane changed. Therefore, 2 days after the addition of red blood cell lysis solution was used in this study, PI-labeled necrosis positive cells were used to observe the effect of Necrostatin-1 on the glial cells. The results showed that Necrostatin-1 could reduce the effect of red cell lysis solution on the necrosis of the glial cells; as the same with Necrostatin-1 can significantly decrease in the number of positive cells in the brain injury and cerebral hemorrhage model in the