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Horseradish Peroxidase Lab Report

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Horseradish Peroxidase Lab Report
In this work, the approach is to develop an electrochemical immunosensor using horseradish peroxidase (HRP) and 3,3’,5,5’- tetramethylbenzidine (TMB) as the enzyme and substrate respectively for cocaine determination. As many studies have shown, TMB3, 4, 5 is commonly used as a chromogen for horseradish peroxidase (HRP). In principle, HRP oxidizes the aromatic amines in the TMB compound by one-electron or two-electron oxidation.4 The produced blue color is a result of either one electron product (diamine) or two electron product (diimine) and one parent diamine.5 Moreover, the formed yellow color after stopping the reaction by sulfuric acid can be identified as two-electron oxidation (diimine). This diimine is stable and electroactive, it
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