Enzyme Lab Report
Enzymes are catalysts that are used to speed up chemical reactions. Adding inorganic catalysts to reactants increase the rate of most chemical reactions. However, all enzymes are different and there are numerous amounts. Many enzymes are essential for life and reactions would not happen rapidly to maintain life with the help of enzymes. Specific enzymes lower the activation energy for specific reactions and shapes. Activation energy is required to start a chemical reaction. This occurs when energy is added to reactants. Amino acid chains are made up by their unique folds which results in formation. Since enzymes are proteins, each one consists of its own amino acid in a specific order. Since hydrogen bonds are weak, they form between amino
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Then a pinch of salt and 1 mL of water was added into each mortar and used the pestle to ground the tissue into a pulp like substance. After it transformed into pulp, 50 μl of the fluid extract was taken to be used for the experiments. Doing so, this evaluated the actions of each catalase in their cells.
Catalase Foam Thickness
50 μl of each tissue extract was added to the cut up tissues, 1 mL of water was added to it and each placed into a separate test tube. After setting up the test tubes, 1 mL of hydrogen peroxide was added to each tube of extract. The thickness of the foam layer that was created in each tube was measured in millimeters.
The effect of Temperature on Catalase
The effect temperature had on the action of each catalase. In the liver cells, the hypothesis was tested based on: Temperature has no effect on catalase activity. First, 50 μl of liver of extract was added along with 1 mL of water in four test tubes. Then placed 1 mL of hydrogen peroxide four other test tubes. Each test tube of catalase was placed in four different degrees celsius (0, 26, 37, 100) for up to 5 minutes. After the time was up, hydrogen peroxide was poured from the second set of test tubes into the first set. Then the thickness of the foam layer of each tubes were …show more content…
When the peroxide was added to the potato and steak extract, 0 mm of foam layer was formed. And when peroxide was added to the liver extract, it created the most foam layer thickness which was 68 mm of foam. When temperature and foam thickness were determined, the results indicated 0 mm of thickness at 0 degrees celsius and gradually increased until it reached 100 degrees celsius. At room temperature, which was 26 degrees celsius the thickness was determined to be 56 mm of foam and at 37 degrees celsius it was 65 mm of foam. The optimum temperature for the catalase activity appeared at 37 degrees celsius. Also, the totally inactivated catalases were the apple and potato because they had no reactivity in foaming at the freezing points, 0 degrees celsius and boiling points, 100 degrees celsius. Errors that might have affected the experiment were, the number of degrees was estimated incorrectly and not as accurate. In order to validate these errors and improve the results, more trials would need to be conducted of the experiment for better
Then a pinch of salt and 1 mL of water was added into each mortar and used the pestle to ground the tissue into a pulp like substance. After it transformed into pulp, 50 μl of the fluid extract was taken to be used for the experiments. Doing so, this evaluated the actions of each catalase in their cells.
Catalase Foam Thickness
50 μl of each tissue extract was added to the cut up tissues, 1 mL of water was added to it and each placed into a separate test tube. After setting up the test tubes, 1 mL of hydrogen peroxide was added to each tube of extract. The thickness of the foam layer that was created in each tube was measured in millimeters.
The effect of Temperature on Catalase
The effect temperature had on the action of each catalase. In the liver cells, the hypothesis was tested based on: Temperature has no effect on catalase activity. First, 50 μl of liver of extract was added along with 1 mL of water in four test tubes. Then placed 1 mL of hydrogen peroxide four other test tubes. Each test tube of catalase was placed in four different degrees celsius (0, 26, 37, 100) for up to 5 minutes. After the time was up, hydrogen peroxide was poured from the second set of test tubes into the first set. Then the thickness of the foam layer of each tubes were …show more content…
When the peroxide was added to the potato and steak extract, 0 mm of foam layer was formed. And when peroxide was added to the liver extract, it created the most foam layer thickness which was 68 mm of foam. When temperature and foam thickness were determined, the results indicated 0 mm of thickness at 0 degrees celsius and gradually increased until it reached 100 degrees celsius. At room temperature, which was 26 degrees celsius the thickness was determined to be 56 mm of foam and at 37 degrees celsius it was 65 mm of foam. The optimum temperature for the catalase activity appeared at 37 degrees celsius. Also, the totally inactivated catalases were the apple and potato because they had no reactivity in foaming at the freezing points, 0 degrees celsius and boiling points, 100 degrees celsius. Errors that might have affected the experiment were, the number of degrees was estimated incorrectly and not as accurate. In order to validate these errors and improve the results, more trials would need to be conducted of the experiment for better