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Meiosis and Mitosis Lab

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Meiosis and Mitosis Lab
AP LAB # 3: MITOSIS AND MEIOSIS

Website to go for quizzes and activities: Print out the quizzes for mitosis and meiosis http://www.phschool.com/science/biology_place/labbench/

OVERVIEW

Exercise 3A is a study of mitosis. You will simulate the stages of mitosis by using chromosome models.You will use prepared slides of onion root tips to study plant mitosis and to calculate the relative duration of the phases of mitosis in the meristem of root tissue. Prepared slides of the whitefish blastula will be used to study mitosis in animal cells and to compare animal mitosis and plant mitosis.

Exercise 3B is a study of meiosis. You will simulate the stages of meiosis by using chromosome models. You will study the crossing over and recombination that occurs during meiosis. You will observe the arrangements of ascospores in the asci from a cross between wild type and mutants for tan spore coat color in the fungus Sordaria fimicola. These arrangements will be used to estimate the percentage of crossing over that occurs between the centromere and the gene that controls that tan spore color.

OBJECTIVES

Section A: Before doing this laboratory you should understand:

* the key mechanical and genetic differences between meiosis and mitosis * the events of mitosis in animal and plant cells * the events of meiosis (gametogenesis) in animal and plant cells

Section B: After doing this laboratory you should be able to:

* recognize the stages of mitosis in a plant or animal cell * calculate the relative duration of the cell cycle stages * describe how independent assortment and crossing over can generate genetic variation among the products of meiosis * use chromosome models to demonstrate the activity of chromosomes during Meiosis I and Meiosis II * relate chromosome activity to Mendelian segregation and independent assortment * calculate the map distance of a particular gene from a chromosome's center for between two

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