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Osmosis
Advanced Placement Biology
®

AP Biology

Lab 1

281
EDVO-Kit # Storage:

Principles & Practice of Diffusion & Osmosis
Store entire experiment at room temperature.

EXPERIMENT OBJECTIVE
The objective of this experiment is to develop an understanding of the molecular basis of diffusion and osmosis and its physiological importance. Students will analyze how solute size and concentration affect diffusion across semi-permeable membranes and how these processes affect water potential. Students will also calculate water potential of plant cells.

EDVOTEK, Inc. • 1-800-EDVOTEK • www.edvotek.com

EVT 080423AM

2
AP
Biology

EDVO-Kit # 281

Principles & Practice of Diffusion and Osmosis

Table of Contents
Lab #

1
Experiment Components Experiment Requirements Background Information Experiment Procedures Experiment Overview PART A. Diffusion and Dialysis PART B. Osmosis PART C. Water Potential PART D. Onion Cell Plasmolysis Study Questions Instructor 's Guidelines Notes to the Instructor Pre-Lab Preparations Experiment Results and Analysis Study Questions and Answers Material Safety Data Sheets

Page 3 3 4

10 11 12 16 20 21

23 24 26 29 31

Advanced Placement (AP) Program is a registered trademark of the College Entrance Examination Board. These laboratory materials have been prepared by EDVOTEK, Inc. which bears sole responsibility for their contents.

EVT 080423AM

1-800-EDVOTEK • www.edvotek.com

EDVO-Kit # 281

Principles & Practice of Diffusion and Osmosis

3

AP

Experiment Components

Biology

Lab #

This experiment is designed for 10 lab groups

Contents A B C D E Orange Indicator Dye, low molecular weight Blue/green dye, high molecular weight 1M Sucrose and 1M NaCl Powdered sucrose NaCl Dialysis tubing

Store entire experiment at room temperature.

1

Requirements
All components are intended for educational research only. They are not to be used for diagnostic or drug purposes, nor administered
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