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Plant Biotechnology Question Papers

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Plant Biotechnology Question Papers
Plant Biotechnology

Long questions * What is in vitro propagation? How can we produce virus-free/pre-basic seed potatoes by thermo-therapy and meristem culture? what is micro propagation?
Describe with illustration the protocol of DAS-ELISA. X2 * Define germplam and cryo-preservation (x2). Describe the steps involved in cryopreservation of shoot tip as a germplasm. X3 * Describe the process of T-DNA transfer and its integration in plant cell by the infection of Agrobacterium. X2 * Describe the method of DNA sequencing by Sanger-Coulson method. X2 * In what principle does Southern Blot work? Describe, how southern blot is performed. X2 * Describe the steps of transformation to produce transgenic plant using Agrobacterium. * Why is apical meristem mostly free from different viruses? Describe different methods of virus elimination from plants. * What are the common DNA polymerases used in DNA cloning? Describe them with their properties. * What is protoplast? How do you isolate and culture protoplast? Describe the methods of protoplast fusion and regeneration of tobacco. * What is soma clonal variation? Write its causes and importance. x2 * What is micropropagation? Describe with ilustrations different types of micropropagation such as meristem culture, shoot tip culture, single node culture, organogenesis and embryogenesis. X2 * What is haploid culture? Why do we use it in plant breeding? Describe with illustrations, anther and microspore culture. X3 * Describe the method of organogenesis and embryogenesis(x2). (Differentiation x1)
Short Answer Questions. * Reporter gene. Significance in gene cloning. * Principle of agarose gel electrophoresis principle used in separation of fragments of nucleic acid. * Describe the process of surface disinfection of an explant. * Describe the different phases of plant tissue culture. Where does sand rooting fit in it? * Describe the steps involved

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    References: Chawla, H.S. (2004) Introduction to Plant Biotechnology. 2nd ed. New Delhi, Oxford & IBH Publishing Co. Pvt. Ltd., pp. 39-56. Dudits, D., Gyorgyey, J. Bogre, L., and Bako, L. (1995) Molecular biology of somatic embryogenesis. In: Thorpe, T.A. ed. In Vitro Embryogenesis in Plants. London, Kluwer Academic, pp. 267-308. Gould, B. (1985) Stata Data Analysis and Statistical Software [Internet], Stata Corporation. Available from: [Accessed 9 May 2012]. Islam, R.M., Mustafi, B.A.A., and Hossain, M. (1996) Socio-Economic Aspects of Fine Quality Rice Cultivation in Bangladesh (Rice Res. Prioritization). New Delhi, Oxford and IBH Publishing Co. Pvt. Ltd., pp. 187-196. Murashige, T., and Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant, 15, pp. 472-493. Pandey, S.K., Ramesh, B., and Gupta, P.K. (1994) Study on effect of genotype and culture medium on callus formation and plant regeneration in rice (Oryza sativa L.). Indian J. Genet. Plant Breed., 54, pp. 293-9. Raina, S.K. (1989) Tissue culture in rice improvement: status and potential. Adv. Agron., 42, pp. 339-398. Sikder, M.B.H., Sen, P.K., Mamun, A.A., Ali, M.R., and Rahman, S.M. (2006) In Vitro Regeneration of Aromatic Rice (Oryza sativa L.). Int. J. Agri. Biol., 8(6), pp. 759760. Steward, F.C., Mapes, M.O., Kent, A.E., and Holsten, R.D. (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am. J. Bot., 45, pp.705-708. Toonen, M.A.J., and De Vries, S.C. (1996) Initiation of somatic embryos from single cells. In: Wang, T.L. and…

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