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Botany Lab

Introduction to Microscope

Circa 1000 AD. = the first vision aid was invented (inventor unknown) called a reading stone.
Circa 1284= Italian, Salvino D’ Armante is credited inventing the first bearable eye glass.
1590= two dutch eye glass makers, Zaccharias Janssen and Hans Janssen experimented with multiple lenses.
1665= English Physicist, Robert Hooke looked at a silver of cork through a microscope lens and noticed some “pores” or “cells”.
1674= Anton Van Leeuwenhoek built a simple microscope with only one lens to examine blood, yeast, insects, and many other tiny objects.
18th Century= technical innovations improved microscope leading to microscopy becoming popular among scientists.
1830= Jospeh Jackson Lister reduces spherical aberration of the chromatic effect.
1931= Ernst Ruska co-invented the electron microscope for which he won the Nobel prize in physics in 1986.
4 kinds of microscope
Compound Microscope
= is a microscope that uses multiple lenses to collect light from the sample and then a separate set of lenses to focus the light into the eye or camera.
Dissecting Microscope (stereo or stereoscopic)
= is an optical microscope variant designed for low magnification observation of a sample using incident light illumination rather than transillumination.
Scanning Electron Microscope (SEM)
= is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. (illumination)
Transmission Electron Microscope (TEM)
= is a microscopy technique whereby a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through.
Parts of the Microscope
Ocular lens= is a type of lens that is attached to a variety of optical devices such as telescopes and microscopes.
Stage= the flat platform where you place your slide. Stage clips hold the slides in place.
Arm= supports the tube and connects it to the base.
Coarse adjustment knobs= large

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