Pre-Chromatographic Isolation Of Baclofen From Su
Weighed accurately about 25.0 mg of baclofen and transferred to 25 ml volumetric flask. Added 20 ml of a 0.001 M aqueous hydrochloric acid solution and sonicated to dissolve and degassed. The resultant solution diluted up to the mark with diluents to obtain 1000 μg/ml. Further stock solution was used to prepare plasma standards to construct the calibration curve.
C. Pre-chromatographic isolation of baclofen from plasma
Prior to chromatography, baclofen from plasma constituents was isolation by adding 0.25 ml of acetonitrile and approximately 25 mg of zinc sulphate crystals to 0.50 ml of plasma. The aged plasma was stored at - 250C. This plasma was thawed at room temperature and 0.50 ml of thawed plasma was pipette into a clean disposable borosilicate test tube. An aliquot of the standard stock solution of baclofen was added to the 0.50 ml of plasma and mixed on a vortex mixer for at least 30 s. A 250µl …show more content…
The mobile phase was filtered through a 0.45 μm membrane filter (Millipore, Bedford, MA, USA) and was then degassed before use. The flow rate of the mobile phase was 0.95 ml/min. DLTH was monitored at 237 nm at a sensitivity of 0.50-0.005 a.u.f.s. The quantitation of DLTH in samples (rabbit’s plasma) was done by comparing the peak height in the sample with a standard calibration curve of DLTH in rabbit plasma.
B. Preparation of stock solution
Weighed accurately about 25.0 mg of diltiazem and transferred to 25 ml volumetric flask. Added 20 ml of a 0.001 M aqueous hydrochloric acid solution and sonicated to dissolve and degassed. The resultant solution diluted up to the mark with diluents to obtain 1000 μg/ml. Further, stock solution was used to prepare plasma standards to construct the calibration curve.
C. Pre-chromatographic isolation of diltiazem hydrochloride from
C. Pre-chromatographic isolation of baclofen from plasma
Prior to chromatography, baclofen from plasma constituents was isolation by adding 0.25 ml of acetonitrile and approximately 25 mg of zinc sulphate crystals to 0.50 ml of plasma. The aged plasma was stored at - 250C. This plasma was thawed at room temperature and 0.50 ml of thawed plasma was pipette into a clean disposable borosilicate test tube. An aliquot of the standard stock solution of baclofen was added to the 0.50 ml of plasma and mixed on a vortex mixer for at least 30 s. A 250µl …show more content…
The mobile phase was filtered through a 0.45 μm membrane filter (Millipore, Bedford, MA, USA) and was then degassed before use. The flow rate of the mobile phase was 0.95 ml/min. DLTH was monitored at 237 nm at a sensitivity of 0.50-0.005 a.u.f.s. The quantitation of DLTH in samples (rabbit’s plasma) was done by comparing the peak height in the sample with a standard calibration curve of DLTH in rabbit plasma.
B. Preparation of stock solution
Weighed accurately about 25.0 mg of diltiazem and transferred to 25 ml volumetric flask. Added 20 ml of a 0.001 M aqueous hydrochloric acid solution and sonicated to dissolve and degassed. The resultant solution diluted up to the mark with diluents to obtain 1000 μg/ml. Further, stock solution was used to prepare plasma standards to construct the calibration curve.
C. Pre-chromatographic isolation of diltiazem hydrochloride from