Scientific Report: Enzyme Lab
1. Prepare a lactase enzyme solution by dissolving one lactase enzyme tablet in 200 ml of water in a clean 250 ml beaker. Stir until the tablet has dissolved. Use labeling tape to label the beaker: “Lactase Enzyme Solution.”
2. Prepare a “denatured” enzyme solution by pouring 20 ml of your enzyme solution into a heat resistant tube. The test tube must have the words “Kimax” or “Pyrex” on it. If it does not, it is not heat resistant and may break! Use labeling tape to label the test tube: “Denatured Enzyme Solution.” Take the test tube to one of the hot plates with boiling water. Place the test tube in the boiling water bath for about 30 minutes. When you get this test tube later, you must use a test tube holder to pick it up. It will be hot!
3. Measure 15 ml of milk and pour it into your small beaker. Use labeling tape to label the beaker: “Milk.” Remember, milk contains the sugar lactose.
4. Prepare a sucrose solution by dissolving a spoonful of sucrose in 100 ml of water in your 3rd beaker. Use labeling tape to label the beaker: “Sucrose Solution.”
PREPARING TEST TUBES
1. Use labeling tape to label one test tube: “A-Milk and Lactase Enzyme Solution.” In this tube combine 2 ml of milk and 1 ml of lactase enzyme solution. Swirl the tube to mix.
2. Label a second tube: “B-Milk and Water.” Combine 2 ml of milk and 1 ml of water in the tube. Swirl to mix.
3. Label a third test tube: “C-Milk and Denatured Lactase Enzyme Solution.” Combine 2 ml of milk and 1 ml of denatured enzyme solution in this tube. Swirl to mix. *You will have to prepare this tube last since you will be waiting for the denatured enzyme solution to heat and then cool a bit.
4. Label a fourth tube: “D-Sucrose Solution and Lactase Enzyme Solution.” Combine 2 ml of sucrose solution and 1 ml of enzyme solution in this tube. Swirl to mix.
5. Label a fifth tube: “E-Sucrose Solution and Water.” Combine 2 ml of sucrose solution and 1 ml of water in this tube. Swirl to mix.
2. Prepare a “denatured” enzyme solution by pouring 20 ml of your enzyme solution into a heat resistant tube. The test tube must have the words “Kimax” or “Pyrex” on it. If it does not, it is not heat resistant and may break! Use labeling tape to label the test tube: “Denatured Enzyme Solution.” Take the test tube to one of the hot plates with boiling water. Place the test tube in the boiling water bath for about 30 minutes. When you get this test tube later, you must use a test tube holder to pick it up. It will be hot!
3. Measure 15 ml of milk and pour it into your small beaker. Use labeling tape to label the beaker: “Milk.” Remember, milk contains the sugar lactose.
4. Prepare a sucrose solution by dissolving a spoonful of sucrose in 100 ml of water in your 3rd beaker. Use labeling tape to label the beaker: “Sucrose Solution.”
PREPARING TEST TUBES
1. Use labeling tape to label one test tube: “A-Milk and Lactase Enzyme Solution.” In this tube combine 2 ml of milk and 1 ml of lactase enzyme solution. Swirl the tube to mix.
2. Label a second tube: “B-Milk and Water.” Combine 2 ml of milk and 1 ml of water in the tube. Swirl to mix.
3. Label a third test tube: “C-Milk and Denatured Lactase Enzyme Solution.” Combine 2 ml of milk and 1 ml of denatured enzyme solution in this tube. Swirl to mix. *You will have to prepare this tube last since you will be waiting for the denatured enzyme solution to heat and then cool a bit.
4. Label a fourth tube: “D-Sucrose Solution and Lactase Enzyme Solution.” Combine 2 ml of sucrose solution and 1 ml of enzyme solution in this tube. Swirl to mix.
5. Label a fifth tube: “E-Sucrose Solution and Water.” Combine 2 ml of sucrose solution and 1 ml of water in this tube. Swirl to mix.