Preview

The Inverted Water Method Lab Report

Better Essays
Open Document
Open Document
1653 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
The Inverted Water Method Lab Report
The inverted water method

MLA citation
BBC News. BBC, n.d. Web. 09 Nov. 2015.
"Factors Affecting Enzyme Activity." Factors Affecting Enzyme Activity. N.p., n.d. Web. 14 Nov. 2015.

Enzymes. Scenario experiment
AIM:
To investigate one factor that affect the distribution of hydrogen peroxide by the catalase in this organism. In this catalase and hydrogen peroxide experiment, we will find out how enzymes act as catalysts by causing chemical reactions to occur. Using a potato and hydrogen peroxide, we can observe how enzymes like catalase work to perform the breaking down, of other substances. We will look at enzymes and catalase and its substrate hydrogen peroxide. Catalase accelerates the breakdown of hydrogen peroxide (H2O2) into water
…show more content…
Plot a graph of rate of oxygen production against concentration of hydrogen peroxide.
Pour the hydrogen peroxide into the test tube that contains the yeast and immediately put the gas syringe bung on the end of the test tube immediately , at the same time start the stopwatch. finish and record results and make a graph. accurate to help make this experiment more accurate i will repeat it 3 times and then use the average of all the results to plot a graph with a line of best fit. i tried keep all the variables except for the concentration of hydrogen peroxide the same for all the experiments. However, it is really hard to keep all the variables precisely the same. For example:
This will slightly affect all the results but as I carried out all the three steps in the same way for all the experiments it should not make any difference to the overall result. safety the way I will make sure I am safe when doing the experiment is I will wear eye protective goggles and protect my clothing from hydrogen peroxide since hydrogen peroxide is harmful and corrosive for the skin I will Report any accidents or spills. don't touch the hydrogen peroxide. Be aware of pressure building
…show more content…
Catalase is an enzyme which breaks down hydrogen peroxide into water and oxygen

how does it work
Catalase works to speed up the decomposition of hydrogen peroxide into oxygen and water. since catalase is an enzyme it lowers the activation energy or how much energy is needed for a reaction to occur.

reaction of hydrogen peroxide
The foam produced in the reaction between hydrogen peroxide and potato is a result of catalase converting hydrogen peroxide into water and oxygen, the bubbles are filled with oxygen. The more bubbles produced the faster catalase is carrying out this reaction, or the more catalase present.

what is the effect of concentration against rate

Results

analysis of results when we increased the concentration of the hydrogen peroxide the rate of reaction increases at a relative even rate until the the concentration of Hydrogen Peroxide reaches about 4 to 5% When the concentration doubled from 3-6% the rate of reaction went up. 1.65 - 2.97 Cm3 Oxygen produced per second, which is an increase of 1.8. I expected the rate of reaction to increase twice when the Hydrogen Peroxide concentration was increased twice because there were twice as many substrate molecules which could join onto the active sites of the

You May Also Find These Documents Helpful

  • Satisfactory Essays

    3. Catalase is an antioxidant enzyme, which is naturally produced in most organisms, used to help break down hydrogen peroxide into oxygen and water. The break down of hydrogen peroxide helps prevent the accumulation of carbon dioxide bubbles in the blood. It is considered to have a great impact because it is capable of decomposing millions of hydrogen peroxide molecules. In addition, it uses the hydrogen peroxide to oxidize harmful toxins within organisms, such as formaldehyde, alcohol, and phenol.…

    • 315 Words
    • 1 Page
    Satisfactory Essays
  • Good Essays

    Depending on the concentration of the catalase which the disk is soaked in, it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas.…

    • 591 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Formal Lab Report 2 Final

    • 1572 Words
    • 7 Pages

    2. Using forceps and scissors cut a small piece of liver and add it to the test tube. Push it into the hydrogen peroxide with a stirring rod. Observe the bubbles.…

    • 1572 Words
    • 7 Pages
    Good Essays
  • Satisfactory Essays

    Methyl Red Voges

    • 271 Words
    • 2 Pages

    The cellular role of a catalase is to enable the breakdown of hydrogen peroxide into water and molecular oxygen.…

    • 271 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Catalase Lab Report

    • 563 Words
    • 3 Pages

    Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach), Brassica oleracea (Broccoli), Solanum tuberosum (Russet Potato), Malus domestica (Apple), and Allium cepa (Onion). The five different catalases from the sources will all be used to catalyze Hydrogen peroxide (H2O2). When hydrogen…

    • 563 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Easy Peasy

    • 401 Words
    • 2 Pages

    4. Different concentrations of hydrogen peroxide solution were added to each test tube using a pipette. (5mL of 6%, 3% and 1.5%)…

    • 401 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    Tiny Bubbles Lab

    • 362 Words
    • 2 Pages

    When yeast and hydrogen peroxide react, the peroxide decomposes into water and oxygen gas causing bubbles to form. These bubbles cause the felt to rise.…

    • 362 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Enzyme Lab

    • 1242 Words
    • 6 Pages

    Title: The Effect of Adjusted Concentration of Hydrogen Peroxide on the change in reaction rate of liver catalase.…

    • 1242 Words
    • 6 Pages
    Good Essays
  • Satisfactory Essays

    lab 6

    • 798 Words
    • 4 Pages

    1. Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…

    • 798 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    Lab 6 enzymes

    • 1000 Words
    • 5 Pages

    Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…

    • 1000 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    Lab 1 again

    • 768 Words
    • 3 Pages

    Figure 1: The reaction rates of enzyme-catalysed reactions of hydrogen peroxide into diluted water depending of the varying percentage concentrations of catalase. Mean rates (±SE) of 5 separate experiments (n=5) are shown. Line of best fit (y = 0.0002x + 0.0038 R² = 0.9154) is presented using the trend line function in Excel.…

    • 768 Words
    • 3 Pages
    Satisfactory Essays
  • Better Essays

    Enzymes combine with reactant molecules (substrate) and bind them closely to one another. The three-dimensional shape of the enzyme molecule must be complementary to the shape of the substrate. Catalysts are not used up in the reaction, and do not furnish energy for the reaction. The reaction can proceed rapidly without much activation energy. Most of these reactions are essentially reversible, and the direction in which the reaction goes depends on the concentration of the reactants in relation to the concentration of the products. The rate of these reactions is controlled by biological catalysts of which are the enzymes. Catalase is an enzyme found in most cells and helps decompose hydrogen peroxide into oxygen and water. There is variation of the effects of temperature upon catalase. Catalysts are not used up in the reaction, and do not furnish energy for the reaction. Catalysts merely affect the rate of the reaction by reducing the amount of activation energy required. Catabolic reactions provide raw materials and starting energy for various anabolic activities. Changes in temperature may change the configuration or shape of an enzyme molecule. If this results in altering the "fit" of the enzyme to its substrate, the speed at which the reaction occurs may be slowed or the reaction may not occur at all. Extremes of high temperatures denature enzymes, which stops their action (Sells, 1999).…

    • 1157 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Enzyme Lab Report

    • 1528 Words
    • 5 Pages

    catalase works to break down hydrogen peroxide. If the pH, temperature, or enzyme concentration changes,…

    • 1528 Words
    • 5 Pages
    Good Essays
  • Good Essays

    I forecast that the more concentrated the hydrogen peroxide is the higher the volume of the foam is and the less concentrated the hydrogen peroxide is the lower the volume of the foam will become.…

    • 619 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Student

    • 1135 Words
    • 5 Pages

    Enzymes are global proteins that act as catalystto biochemical reactions in living cells. A catalyst alters the speed of a chemical reaction without itself undergoing any permanent change. An enzyme is a biological catalyst that increases the rate of chemical reaction by lowering the level of activation energy necessary to start the reaction. Enzymes speed up these reactions by bringing the reactants into close proximity and facilitating their interaction. Liver contains a specific enzyme called catalase. Enzyme molecules have a small region that is functional, called the active site. In an enzyme-catalyzed reaction, the substrate binds to the active site and forms enzyme-substrate complex with the enzyme. The enzyme then breaks the bonds in the substrate. The product ofthe reaction then leaves the enzyme, which remains unchanged after the reaction. Catalase in an enzyme produced by our liver to break down hydrogen peroxide – a…

    • 1135 Words
    • 5 Pages
    Better Essays