Preview

Types of DNA Technology

Good Essays
Open Document
Open Document
1361 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Types of DNA Technology
DNA Technology: Biotechnology
1) Selective breeding
a.
2) Genetic engineering – humans tinker with organisms genes
a. Cloning –take haploid cel and replace with a diploid nucleus (comes from organism whos traits you want to duplicate.
b. Recombinant DNA – DNA from 2 or more sources. Done by Euk cells during Meiosis. Always from same molecule.
3) Biologists first started doing recombinant DNA from a prok cell and combined it with another prok cell because it was easier.
4) DNA from a euk cell and combine it with DNA from a prok cell
Features of bacteria that make them useful subjects for recombinant DNA technicians:
1) Short generation time. Divide fast
2) Easy uptake of DNA from environment (transport from environment) [Fred Griffith transformation did this].
a. Many have Calcium driven DNA pumps. (especially small DNA molecules)
b. Electroporation – shock DNA to open pores in plasma membrane so DNA can enter cell
c. Have small DNA molecules w/ 0 plasmids 1 ori and carry accessory genes
3) Restriction endonucleases attack DNA molecule from inside.
Exonucleases – chop off 1 nucleotide at a time at the ends of the DNA molecule. Not in abundant in prokaryotes
Bacterial DNA gets methylated – restriction enzymes cut only unmethylated DNA
Bacterial DNA gets bacteria add CH3 to Cytosine or Thymine to protect their own enzymes Over time they have to choose to be unmethylated or methylated to cut DNA
2 types of restrictiom enzymes used to cut plasmids
Restriction enzymes that open up plasmids at a restriction site 4-8 base pairs long Palindromic CCCGGG Make staggered cuts
Plasmids are opened at one restriction site. Foreign Dna treated with same restriction enzymes
Naïve bacteria have no plasmids.
Makes:
Original plasmid
Plasmid with wrong foreign DNA fragment
Plasmid with gene of interest
-mix with naïve bacteria get you types of bacteria
Results of recombined plasmid exposed to naïve bacteria- the 4 results:
1: naïve

You May Also Find These Documents Helpful

  • Good Essays

    Ap Bio Final Study

    • 3862 Words
    • 16 Pages

    Populations A may undergo instantaneous speciation by the doubling of its chromosome numbers (polyploidy). Rough endoplasmic reticulum, golgi complex, vesicle, plasma membrane stabilizing selection…

    • 3862 Words
    • 16 Pages
    Good Essays
  • Good Essays

    Bio341 Unit 1 Essay

    • 718 Words
    • 3 Pages

    9. What are the two ways in which meiosis brings about genetic recombination and which stage or stages of meiosis is responsible?…

    • 718 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    D. All of the genes controlling the traits behaved as if they were on different chromosomes.…

    • 495 Words
    • 1 Page
    Satisfactory Essays
  • Good Essays

    Nt1310 Final Exam

    • 1248 Words
    • 5 Pages

    b. The DNA separates into two single strands instead of remaining as a double helix.…

    • 1248 Words
    • 5 Pages
    Good Essays
  • Good Essays

    2) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation…

    • 4889 Words
    • 20 Pages
    Good Essays
  • Good Essays

    Dna Sci/230

    • 494 Words
    • 2 Pages

    3. Describe each stage of the flow of information starting with DNA and ending with a trait.…

    • 494 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Chapter 10 Gene Technology

    • 2264 Words
    • 10 Pages

    Plasmids or viruses can serve as _______________ to carry foreign DNA into the host cell.…

    • 2264 Words
    • 10 Pages
    Powerful Essays
  • Satisfactory Essays

    dna worksheet

    • 380 Words
    • 2 Pages

    3. Describe each stage of the flow of information starting with DNA and ending with a trait.…

    • 380 Words
    • 2 Pages
    Satisfactory Essays
  • Powerful Essays

    Bio Midterm 1 Review

    • 1812 Words
    • 8 Pages

    6. Two identical DNA molecules in a eukaryotic cell that are created through DNA replication are?…

    • 1812 Words
    • 8 Pages
    Powerful Essays
  • Good Essays

    f. The creation of a knockout mouse uses the process of __ recombination___ to replace DNA with an engineered construct…

    • 814 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    1.3.1 response

    • 620 Words
    • 3 Pages

    Visit the list of restriction enzymes found at the bottom of the page on http://en.wikipedia.org/wiki/Restriction_enzymes. Find the unique sequence (restriction site) that is recognized by EcoRI and by HindIII. Write the double-stranded sequence below and draw an arrow between the base pairs to indicate where the enzyme would make its cut.…

    • 620 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Week 5 Study Guide

    • 792 Words
    • 4 Pages

    D. What are the two stages required to go from DNA to trait formation and where does each stage occur in a cell?…

    • 792 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Week 3 Activity

    • 464 Words
    • 3 Pages

    7. During which state of the cell cycle does a cell duplicate its genetic material?…

    • 464 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Mastering Biology

    • 2505 Words
    • 11 Pages

    - two cells, each with the same amount of genetic material and the same genetic information…

    • 2505 Words
    • 11 Pages
    Good Essays
  • Good Essays

    Gene Transfer Lab Report

    • 939 Words
    • 4 Pages

    Escherichia coli is a bacterium that can affect our health or even kill. Like most bacteria, E. coli is able to change and progress into different forms based on genetic changes that they can go through. One example of this genetic change is shown in the E. coli becoming immune to ampicillin is blood infections. Because ampicillin has been used so frequently to fight the symptoms of an E. coli infection, the bacteria has been able to change itself genetically by producing more of an inhibitor resistant TEM in order to continue it’s genetic line and reproduce causing infections in humans (Walters-Toews, et al. 2011). Another example from the science field would be an experiment that suggests that E. coli is not only becoming resistant to ampicillin, but also other antibiotics including Cotrimoxazole and Cefuroxime (Renal & Urology News, 2007). This experiment is meant to prove that through genetic transfer using plasmid DNA, the E. coli can become bioluminescent and immune to the ampicillin. By adding plasmid DNA to the E. coli cells, the genetic composition of the cells will be different. I predict that the E. coli cells containing no ampicillin will be able to grow colonies. I also predict that the plates with plasmid DNA will show signs of bioluminescence. The plate with ampicillin present with no plasmid DNA will not be able to grow colonies and will not be capable of bioluminescence.…

    • 939 Words
    • 4 Pages
    Good Essays

Related Topics