Enterobacteriaceae (enterics) -Gram neg., rod shaped fermenting sugars to produce lactic acid. Ex: Salmonella, E. coli, Shigella…
In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is a fermenter they will use the sugars to make ATP. If the bacteria is a fermenter of lactose/sucrose the EMB plate we used will “clearly differentiate between the colonies of lactose fermenting and non-fermenting microbes. In the same medium sucrose was also included to differentiate between coliforms that were able to ferment sucrose more rapidly than those that were unable to ferment sucrose” (Cheeptham & Lal, 2007).…
This experiment is aimed to examine the effects of environment such as Oxygen, Temperature, pH and Osmotic Limitations on the growth of a various kind of bacteria.…
This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose, glucose, and lactose), the Urease Test, the Fermentation of Lactose Test, the Sulfide Indole Mobility (SIM) Test, the Nitrate Reduction Test, the Protein Hydrolysis Test, the Catalase Test, and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli, Bacillus cereus, the unknown, Proteus vulgaris, Staphylococcus epidermis, Enterobacter aerogenes, the control, and Pseudomonas fluorescens. The microbes tested during these various tests were looking for which would: reduce sulfur/produce sulfate, produce indole, or possess motility, reduce nitrate, and contain protease, catalase and oxidaase.…
Give examples of bacteria classified as Archeabacteria and Eubacteria. Answer: Archeabacteria: Methanogens – “methane makers”; Extreme halophiles – “salt lovers”; Extreme thermophiles – “heat lovers” Eubacteria: spirochetes; chlamydias; proteobacteria; Gram-positive bacteria; cyanobacteria…
The H2S Production test was used to test only the Unknown Gram-negative bacteria. One colony from the Unknown 13B plate was used to inoculate a SIM Agar Deep Tube. After the culture was inoculated, it was placed in an incubator at 37ºC for 48 hours. A positive H2S Production test would have showed a black precipitate along the stab line in the media, which indicated that ferrous ammonium sulfate combined with gas to form the black precipitate (Pearson’s 2011). A negative H2S Production test would have showed no black precipitate along the stab line in the media (Pearson’s 2011).…
Microorganisms are plentiful and widespread in the environment. In this lab, we undertook to determine the differences in the agars being used and the different colony count observed. After taking four different samples of microbes from the environment and swabbed them in two different plates one with nutrient agar and the other with sabouaud dextrose agar. After the microbes had incubated for 48 hours no results were discovered from the swabs we had taken from the environment. This lab further investigates the different swabs from the environment other group got and the different results discovered from the agars being used and the colony count observed.…
E. coli belongs to the phylum of Proteobacteria, it is in the class of gammaproteobacteria and the family of enterobacteriaceae. There are around 200 different E.coli serotypes. When stained, E. coli appears as gram-negative bacilli. It appears in pairs or singly. E. coli is non spore forming, catalase positive, oxidase negative and facultatively anaerobic or aerobic. E. Coli can be motile by peritrichous flagella or…
The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature, pH level, and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria can also be used in many other areas from better crop production through microbial pesticides to biological warfare. Their uses are endless as are their abilities to evolve and adapt to changing environments. That is why it is so important to be able to identify microorganisms. This study was conducted using techniques and experiments learned in microbiology lab that were used to classify the two unknown bacteria.…
Ms. AG Case Analysis Paternalism Vs. Autonomy One of the fundamental principles of healthcare ethics is patient autonomy. The struggle, however, is determining how much independence the patient has. Is it appropriately and ethically right to allow an individual to have complete autonomy?…
II. Background: There are four different types of media used to grow microbes. They consist of Enriched media, Nutrient media, Selective media, and differential media.…
In this laboratory exercise explore the differences of microorganism and continue our use of specialized media and use some biochemical testing.This report discloses the basic laboratory instruments will be used in each of our practices .It is of great importance to recognize and identify the different instruments and laboratory tools, because in this way will we be able to use them properly and also to call them by name and know why.…
1.1 Bactetria are: Bacteria are organisms made up of just one cell. They are capable of multiplying by themselves, as they have the power to divide into different shapes. Their shapes vary, and that’s how they are used to separate them into groups. Usually a few micrometres in length. Bacteria are present in most surroundings on the planet, growing in soil, acidic hot springs, radioactive waste, water, and deep in the Earth's crust, as well as in organic matter and the live bodies of plants and animals, providing outstanding examples of mutualism in the digestive tracts of humans, termites and cockroaches.…
Oxygen in the body is depleted by aerobic organisms; creating an ideal environment for the new creation of anaerobic organisms. Anaerobic organisms from the gastrointestinal tract and respiratory system transform carbohydrates, lipids, and proteins yielding organic acids (propionic acid, lactic acid), and gases, (methane, hydrogen sulphide, ammonia). The process of microbial proliferation within a body is referred to as putrefaction and leads to the second stage of decomposition.…
Address Applied Biotechnology Research Group, School of Life Sciences, University of Westminster, United Kingdom Corresponding author: Keshavarz, Tajalli (T.Keshavarz@westminster.ac.uk) and Current Opinion in Microbiology 2010, 13:321–326 This review comes from a themed issue on Ecology and Industrial Microbiology Edited by Erick Vandamme Available online 12th March 2010 1369-5274/$ – see front matter # 2010 Elsevier Ltd.…