BE8504 Soil Science Experiment 8 - Loss On Ignition Singapore Polytechnic DEWT/FT/1A/01 Aim: To determine the concentration of organic contaminants in soils by using the Loss in ignition method. Introduction: The quantity of organic matter in soil is determine by either direct or indirect methods. Direct method target the destruction of all organic matter‚ after which the loss in weight of soil is taken as the organic content. Two methods are commonly used to achieve destruction of organic
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Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from
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Pre-Lab 1 Assignment Quantitative Analysis of Biomolecules Biochemical analysis involves the characterization of biomolecules within a sample using appropriate laboratory techniques. There are two principal approaches: 1. Qualitative analysis – where a sample is analyzed to determine whether a biomolecule is present or absent. As an example‚ a blood sample might be analyzed for a specific antibody or a bacterial cell might be probed for a nucleic acid sequence. 2. Quantitative analysis
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Percent Copper in Brass Background The scientific concepts involved in this lab include Spectroscopy‚ Beer’s law‚ Calibration curve‚ concentration‚ and electronic transitions. The main objective of this experiment is to see how the percent composition of brass can be determined to verify the properties influenced by copper and zinc. Brass is a generic term for alloys of copper and zinc. The main technique used in this experiment is Spectroscopy. The three equations used in this lab are: Y = mx
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What physical evidence did you find at the victim’s home? Matches Space heater Bear bottles The girlfriends wallet Describe the process that you used to take a sample of the puddle-shaped burn pattern on the floor. Why did you also take samples from the portion of the floor that was not burned? To take a sample of the burned floor we scraped the burnt wood will we got loose pieces of it. We did the same to take a sample of the wood that had not been burned. We took a sample of the burned
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Separating Water Soluble and Acetone Soluble Inks through Chromatography Abstract: Chromatography is a term used to define a set of laboratory techniques for the separation of mixtures. Through Chromatography we analyzed complex mixtures. In our recent lab we analyzed complex mixtures. The purpose of this lab was to find whether the water soluble or acetone soluble liquid traveled farther. In our recent lab we measured the distance traveled by the different colored water and acetone soluble
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Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
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Phagocytosis in Tetrahymena Does Cigarette Extract Alter the Rates of Food Vacuole Formation? Amber Ellis 11/20/2014 Bio 1130 50 Jennifer Dubberke Abstract Does Cigarette Extract Alter the Rates of Food Vacuole Formation? The objective of the experiment is to visualize tetrahymena cells and vacuole formation‚ quantify tetrahymena and vacuole formation over a defined time period. Also‚ investigate if cigarette extract alters the rate of food vacuole formation. The materials needed in completing
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Introduction In a chromatographic separation process‚ different kinds of functional groups and number of functional groups of molecules will determine the separation. Depending on what kind of solute‚ solvent‚ and adsorbent used‚ molecules will experience the interaction during dynamic equilibrium. On a TLC plate‚ capillary tube is used to transfer o- and p- hydroxyacetopheone‚ taking advantage of capillary force to make small spotting. A 30:70 ether acetate: petroleum ether is used as a solvent
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