Bleaching of crude palm oil (CPO) Bleaching of crude palm oil will be carried out using a mass ratio of 1 synthesized adsorbent to 11 CPO. The CPO in bottle is well shaken before pouring into beaker with an amount of 110 grams. Magnetic stirrer is put into the beaker contains CPO and continuously stirred until it reaches 100 °C. After that‚ 6 drops of phosphoric acid is added into CPO followed by the addition of 10 grams synthesized adsorbent. The adsorbent must be poured slowly to ensure it mixed
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes‚ and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid
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LAB 4 – SEPARATION OF A MIXTURE OF SOLIDS Purpose: To determine and execute the separation of mixture of solids through different means. Examples are magnetisms‚ evaporation‚ and filtration. Observations and Experimental Data: Data Table 1: Experiment Data Grams Percent of mixture Iron filings 1.4 g 31.1 % Sand 1.3g 28.9 % Table salt 1.0g 22.2% Benzoic acid .8g 17.8 % Total 4.5g 100% Questions: A. How did your proposed procedures or flow charts
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PROCEDURE 1) A standard was prepared in solvent and was placed into sample holder for the purpose of calibration curve. 2) The liquid sample was also prepared in a similar manner and placed into sample holder. 3) The UVProbe Software was opened and the method(M) was set to wavelength of 800-0.0 at medium speed for measuring liquid sample absorbance ‚ and the method was saved in a file according to sample name. 4) The standard for baseline measurement was run. (The first run using an empty cuvette
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(1) Answer to Question in lab manual. NO introduction/background etc. 1. What is the retention time for caffeine? Retention time for caffeine : 4.149+4.142+4.152+4.1454 = 4.15 2. What is the retention time for benzoic acid? Retention time for caffeine : 2.651+2.817+2.857+2.9274 = 2.81 3. How are the retention times for caffeine and benzoic acid related to their chemical structure and the nature of the chromatographic
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Objective: The objective of the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the
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Chromatography is the separation of compounds to allow us to see the different colours of that compound‚ for instance we may use chromatography in a school lab to see the real colour compounds of plants and grass‚ by using chemicals such as acetone we it will allow the object to break compounds and thus we can see the true colour of the grass. In today’s society there are many real world applications which use the chromatography technique in their line of work‚ an example of one of these applications
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