Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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Observing Bacteria Kelli Jo Simco Microbiology Due: 2/8/13 Abstract: Microscopes are fragile instruments that must be handle with extreme caution as they can produce high quality results when observing the smallest specimens on earth. A microscope must be properly cleaned before use and storage. The different objectives allow for a range of observations. At the highest objectives‚ the resolution can easily be lost which is why the oil immersion lens is used to minimize refraction. While
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Anthrax-Bacillus anthracis Anthrax is caused by the bacterium called Bacillus anthracis which is a spore forming‚ rod shaped bacterium. Central spores develop under all growth conditions except the living body of the host. It is classified as Bacilli. The most common form would be the cutaneous form. however as we saw during the 2011‚ terrorist attacks‚ the pulmonary form is very real and a serious threat to life. It affects the lungs and the GI tract. The last form is the
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In case the cell line has to be stored it should be at -80 C or below. Te cell lines that should be used for the test should be free from mycoplasma before use the stock culture should be tested for the absence of mycoplasma. The pH of the culture medium should be maintained in the range of 7.2-7.4. The qualitative and quantitative assessment of cytotoxicity has to be tested. The test should be performed on a sub-confluent mono
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crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at 35º C Organisms ATCC Growth Colour Escherichia coli 25922 + red Proteus mirabilis 12453 + colourless Salmonella typhimurium 14028 + colourless Streptococcus faecalis 29212 - or partial Uses Acting as a visual ph indicator‚ the agar distinguishes
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Objectives: • To measure the different phases of growth of Escherichia coli through absorbance reading and viable count measurements • To plot the growth curve of Escherichia coli Methodology: [pic] [pic] [pic] Results and Discussion: In the experiment‚ the different growth phases were observed through the analysis of the absorbance of broth with inoculated organism (E.coli). There was no
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Anthrax is a lethal disease that is caused by gram-positive‚ rod-shaped bacteria known as Bacillus anthracis. Many researchers believe that this disease originated in Egypt and Mesopotamia in 1250 BC. However‚ there were also other regions during this time period who were acquainted with anthrax‚ such as Ancient Greece and Rome. Many illustrations and ancient writings by famous scholars of those times depicted this disease. For example‚ the author Homer illustrated anthrax in his poem Illiad from
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Growth and One Step Burst - T7 Phage I. INTRODUCTION: These experiments helped us learn the factors that were involved in the growth of the bacteria that increased our study towards their genetic‚ physical and metabolic characteristics. We used Escherichia coli and Bacteriophage T7 to identify and analyze their identical life cycle and replication that was involved in their process of growth. As‚ growth for any bacteria means it multiplying by increasing in its size and quantity‚ which can easily
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B.TECH MBA(BIOTECH} ROLL NO. =63 INTRODUCTION Bacillus thuringiensis is a Gram-positive‚ soil-dwelling bacterium of the genus Bacillus. Additionally‚ B. thuringiensis also occurs naturally in the gut of caterpillars of various types of moths and butterflies‚ as well as on the dark surface of plants. B. thuringiensis was discovered 1901 in
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types of bacteria. Procedure: 1. Focus the microscope on a slide containing the letter ‘e’. 2. Focus the microscope on 6 other prepared slides. 3. Focus the microscope on slides Bacteria spirillum‚ Bacteria coccus‚ and Bacteria bacillus. 4. Prepare a fresh yoghurt slide for viewing under the microscope‚ and then compare what you see to what you see on your prepared slide from the lab kit. Data/Observations 1. The microscope was focused on the “e” slide and the slide
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