E.Coli Growth Curve
Objectives:
• To measure the different phases of growth of Escherichia coli through absorbance reading and viable count measurements • To plot the growth curve of Escherichia coli
Methodology:
[pic]
[pic]
[pic]
Results and Discussion:
In the experiment, the different growth phases were observed through the analysis of the absorbance of broth with inoculated organism (E.coli).
There was no viable cell counting done with the plates because of the failure to count and record the number of viable cells after a 24-hour incubation period. The measurement of absorbance may be used to analyze bacterial growth since the absorbance or the measure of the turbidity is directly proportional to the number of cells. Turbidity results from the deflection of light by bacterial cells present, thus, the more turbid the broth is, the more bacterial cells are present and the higher the absorbance reading is.
There are four characteristic phases of growth of typical bacteria in batch culture (closed system): lag phase, log phase, stationary phase and death phase. The lag phase is known as the adjustment period of the bacteria. There is lag, hence the name, before the resume of cell division.
During this phase, there is only an increase in cell size. However, towards the end of the lag phase, after the bacteria have already adjusted enough, cell division happens. In the graph, the lag phase is from time 30 until 150 minutes. The early lag phase or adjustment period of the cell as seen from the graph appears to have constant absorbance measurement. If we look very closely, there is still an increase in cell number but during the early lag phase, there is only very minimal. Towards the end of the lag phase, from time 60 to 150 minutes, there is a more significant increase in microbial population.
The next phase, log phase or exponential
• To measure the different phases of growth of Escherichia coli through absorbance reading and viable count measurements • To plot the growth curve of Escherichia coli
Methodology:
[pic]
[pic]
[pic]
Results and Discussion:
In the experiment, the different growth phases were observed through the analysis of the absorbance of broth with inoculated organism (E.coli).
There was no viable cell counting done with the plates because of the failure to count and record the number of viable cells after a 24-hour incubation period. The measurement of absorbance may be used to analyze bacterial growth since the absorbance or the measure of the turbidity is directly proportional to the number of cells. Turbidity results from the deflection of light by bacterial cells present, thus, the more turbid the broth is, the more bacterial cells are present and the higher the absorbance reading is.
There are four characteristic phases of growth of typical bacteria in batch culture (closed system): lag phase, log phase, stationary phase and death phase. The lag phase is known as the adjustment period of the bacteria. There is lag, hence the name, before the resume of cell division.
During this phase, there is only an increase in cell size. However, towards the end of the lag phase, after the bacteria have already adjusted enough, cell division happens. In the graph, the lag phase is from time 30 until 150 minutes. The early lag phase or adjustment period of the cell as seen from the graph appears to have constant absorbance measurement. If we look very closely, there is still an increase in cell number but during the early lag phase, there is only very minimal. Towards the end of the lag phase, from time 60 to 150 minutes, there is a more significant increase in microbial population.
The next phase, log phase or exponential
References: Abedon, S. P. (1998, April 3). Microbial Growth. Retrieved from http://www.mansfield.ohio-state.edu/~sabedon/biol2025.htm Black, J.G. (1996). Microbiology. Principles and Applications. Third Edition. Prentice Hall. Upper Saddle River, New Jersey. pp. 136-140, 151-153. ----------------------- Refrigerate plates after 24 hours of incubation then count bacterial cells Refrigerate plates after 24 hours of incubation then count bacterial cells