Introduction Enzymes are catalytic proteins. The purpose of a catalyst is to speed up metabolic reactions by lowering the free energy of activation or activation energy. Activation energy is known as the amount of energy needed to push the reactants over an energy barrier‚ so that the downhill part of the reaction can begin (Campbell 151). In an enzyme catalyzed reaction‚ the enzyme binds to its substrate‚ which is the reactant an enzyme acts on. In the reactions‚ the enzymes are very specific
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Figure 3 Superoxide radical scavenging activity. Values are mean ± SEM (n = 3). ABL‚ lyophilized aqueous extract of A. bilimbi fruits; ABP‚ petroleum ether fraction of ABL; ABE‚ ethyl acetate fraction of ABL; ABB‚ butanol fraction of ABL; ABW‚ aqueous fraction of ABL; QU‚ quercetin. 3.3.5 Nitric oxide radical scavenging activity Lyophilized aqueous extract of Averrhoa bilimbi fruits (ABL) and its different solvent fractions exhibited a dose dependent inhibition of nitric oxide radicals. The IC50
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Ayaan Nadeem SBI 4UO-A Tuesday‚ February 27‚ 2013 Ms. Balmer Pineapple Jelly Enzyme Lab Discussion After completing the Pineapple Jell-O Enzyme lab‚ the final results were that the canned pineapple formed the jelly while the fresh pineapple did not. Pineapple In order for this to have occurred‚ there has to be a comparison between fresh and canned pineapple in terms of their physical and chemical properties. The physical properties of fresh pineapple are that it is sweet‚ ripe and raw.
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I.. "Osmosis and Diffusion - A Tutorial." Memorial University. N.p.‚ n.d. Web. 2 Apr. 2013. http://www.mun.ca/biology/Osmosis_Diffus Smith. "Investigating the effect of concentration of blackcurrant squash on osmosis in chipped potatoes | Nuffield Foundation." Nuffield Foundation |. N.p.‚ 24 Nov. 2011. Web. 2 Apr. 2013. (Figure 1.0) http://www.nuffieldfoundation.org/practical-biology/investigating-effect-concentration-blackcurrant-squash-osmosis-chipped-potatoes Steinberg‚ June. "osmosis." ESR
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds up the rate of enzyme activity and any increase
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Lab Report: Toothpickase and Enzymes September 19‚ 2012 Olivia DePhillips Lab Partner: Joy Morgan Meyers Signature: Introduction Enzymes are biological molecules that catalyze chemical reactions. In enzymatic reactions‚ the molecules at the beginning of the process‚ called substrates‚ are converted into different molecules‚ called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. Like all catalysts‚ enzymes work
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TEMPERATURE ON CATALASE ACTIVITY AIM The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase’s different attributes. HYPOTHESIS In this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a little activity would take place around
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Period 5 9/4/12 Laboratory 2 Report: Enzyme Catakysis 1. Title • The effect that temperature‚ pH‚ time‚ enzyme concentration‚ and substrate concentration has on Enzyme Catalysis. 2. Abstract • Assess the general functions and activities of enzymes. Analyze the relationship between the structure and function of enzymes. Understand the concept of initial reaction rates of enzymes. Change the temperature‚ pH‚ enzyme concentration‚ substrate concentration‚ and
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Abstract For this experiment we tested the effects of different adrenalin concentrations (0.001%; 0.01%; 0.1%) on the daphnia flea. The daphnia was placed on a slide with cover slip in order to examine its heartbeat by using the microscope. Aim In this Lab we will study the effect of different concentrations of adrenalin on the heart rate daphnia‚ the water flea. It is expected to recognize a threshold of the daphnia in its reaction the three different adrenalin concentrations‚ in terms of heart rate
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