solutions‚ Kovac’s reagent‚ zinc dust‚ hydrogen peroxide‚ API 20E incubation tray‚ Bunsen burner‚ oxidase reagent and bacteria Methods: The method used for the API (analytical profile index) 20E System was the aseptic technique was used for the inoculating loop to get a unknown colony from the streak plate or pure culture slant. A small amount was smeared over the filter paper and several drops of oxidase reagent were added and the color change was noted. Another loop-full of bacteria was added to
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DELA CRUZ‚ CYNTHIA GRACE C. 2008-13749 FS 125 March 10‚ 2012 ANTIMICROBIAL AGENTS 1. Introduction Food preservation has long been practiced since the olden times with processes such as heating ‚ drying‚ fermentation and refrigeration. In spite of this‚ physical methods are not enough to eliminate all microorganisms found in foods. Therefore‚ antimicrobial agents are needed to destroy these foodborne pathogens‚ so that the food will have a longer shelf-life. As technology progresses‚ improvements
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rays‚ and immobilization of toxic ions. There are several types of melanin; eumelanin and pheomelanin (found mostly in animals as L-DOPA)‚ Neuromelanin (found in brain as dopamine‚ norepinephrine‚ and serotonin)‚ Allo Melanin (found in plants‚ as catechol‚ and pyrogallol)‚ Pyomelanin (found in fungi or bacteria as homogentisic acid) Melanin is important to this experiment because‚ the structure of melanin is not fully known. With more knowledge about this chemical‚ and the way it function‚ we would
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February 24‚ 2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test‚ an oxidase test‚ a gram stain‚ a hanging drop slide‚ and morphology identification. The unknown bacterium‚ which was contaminated with Serratia marcescens‚ was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria
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Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase‚ catalase‚ lactose and sucrose fermentation‚ Kugler/iron agar‚ nitrate reduction‚ gelatin hydrolysis‚ starch hydrolysis‚ manitol salt‚ MR-VP‚ citrate‚ bile esculin‚ indole‚ urease‚ DNase‚ and coagulase. Material & Methods The tests performed
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Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
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I had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
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Throughout the week in the toxicology lab‚ I was acquainted with distinctive sort of tests and I got the opportunity to find out about the following: 1- hTSH test (Human thyroid stimulating Hormone): - Add calibration and +/- controls‚ before starting the punching process. - Add hTSH assay buffer + stock solution. - Put the plate on a fast quick shaking for 10mins‚ and afterward 4 hours moderate shaking at room T° -Add washing solution. -Add enhancement solution‚ and read the result.
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Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation of Lactose Test‚ the Sulfide Indole Mobility (SIM) Test‚ the Nitrate Reduction Test‚ the Protein Hydrolysis Test‚ the Catalase Test‚ and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli‚ Bacillus cereus‚ the unknown‚ Proteus vulgaris‚ Staphylococcus epidermis‚ Enterobacter aerogenes‚ the control‚ and Pseudomonas fluorescens. The microbes tested during these
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INTRODUCTION Many infectious diseases caused by infectious agents‚ including bacteria‚ fungi‚ viruses and parasites‚ have plagued human existence CITATION Kon97 \l 1033 (Koneman‚ 1997). Thus the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate
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