February 24‚ 2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test‚ an oxidase test‚ a gram stain‚ a hanging drop slide‚ and morphology identification. The unknown bacterium‚ which was contaminated with Serratia marcescens‚ was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria
Premium Microbiology Bacteria Gram staining
Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase‚ catalase‚ lactose and sucrose fermentation‚ Kugler/iron agar‚ nitrate reduction‚ gelatin hydrolysis‚ starch hydrolysis‚ manitol salt‚ MR-VP‚ citrate‚ bile esculin‚ indole‚ urease‚ DNase‚ and coagulase. Material & Methods The tests performed
Premium Enzyme Staphylococcus Staphylococcus aureus
Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
Premium Enzyme
I had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
Premium Bacillus Bacteria Enzyme
Throughout the week in the toxicology lab‚ I was acquainted with distinctive sort of tests and I got the opportunity to find out about the following: 1- hTSH test (Human thyroid stimulating Hormone): - Add calibration and +/- controls‚ before starting the punching process. - Add hTSH assay buffer + stock solution. - Put the plate on a fast quick shaking for 10mins‚ and afterward 4 hours moderate shaking at room T° -Add washing solution. -Add enhancement solution‚ and read the result.
Premium Chemistry Oxygen Water
Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation of Lactose Test‚ the Sulfide Indole Mobility (SIM) Test‚ the Nitrate Reduction Test‚ the Protein Hydrolysis Test‚ the Catalase Test‚ and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli‚ Bacillus cereus‚ the unknown‚ Proteus vulgaris‚ Staphylococcus epidermis‚ Enterobacter aerogenes‚ the control‚ and Pseudomonas fluorescens. The microbes tested during these
Premium
INTRODUCTION Many infectious diseases caused by infectious agents‚ including bacteria‚ fungi‚ viruses and parasites‚ have plagued human existence CITATION Kon97 \l 1033 (Koneman‚ 1997). Thus the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate
Premium Microbiology Bacteria
were gram positive cocci arranged in irregular clusters. I ran a catalase test on both organisms; organism A was catalase positive while organism B was catalase negative. I also performed a cytochrome oxidase test on both organisms; again‚ organism A was oxidase positive while organism B was oxidase negative. Then I decided I would run the biochemical tests on organism B. I observed that organism B was non-motile and did not produce endospores. With these results‚ I narrowed down the possible species
Premium Bacteria Streptococcus Agar plate
ABSTRACT In todays world of ever evolving genetic mutations the incidence of drug resistant forms of microorganisms are on the rise. The importance of identifying these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine
Premium Microbiology Bacteria Staining
Medical Microbiology II - Laboratory Report 1 Aim: To successfully identify and differentiate Neisseria Species and Moraxella catarrhalis; i. detail the microscopic and cultural characteristics of the Neisseria spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral
Premium